Product Name:
ATG5
Product Number:
ab-nn212-1
Target Full Name: Autophagy protein 5
Target Alias: APG5-like; ASP; hAPG5
Product Type Specific: Autophagy protein pan-specific antibody
Antibody Code: NN212-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9H1Y0
Protein SigNET: ATG5
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Synthetic peptide patterned after mid-region of human ATG5
Production Method: Peptide Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Predicted molecular mass at ~32.4 kDa. Observed molecular masses at ~48-60 kDa based on ATG12-ATG5 heterodimer.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human.
Antibody Specificity: Very high
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Related Product 9: ATG4D expression antibody (Cat. No.: AB-NN211-2)
Related Product 10: ATG5 expression antibody (Cat. No.: AB-NN212-2)
Scientific Background: ATG5 (Autophagy-related protein 5) is an E3-like ligase in the autophagy pathway, and essential for autophagosome formation and elongation. It is important for cellular homeostasis, quality control, pathogen resistance, and immune regulation. It forms a complex with ATG12 and ATG16L1 to facilitate the conjugation of LC3-I to PE to form LC3-II. ATG5 is involved in autophagic vesicle formation. It conjugates with ATG12 in a ubiquitin-like conjugating system that involves ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme. The ATG12-ATG5 conjugate acts as an E3-like enzyme, which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. ATG5 is involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. ATG5 can also engage in autophagy-independent processes, such as promoting apoptosis by interacting with FADD (Fas-associated protein with death domain), regulating antiviral immune responses, and influencing lymphocyte development. It is vital for resistance against intracellular pathogens, including Toxoplasma gondii and L. monocytogenes. Variants in the ATG5 gene have been linked to autoimmune diseases like systemic lupus erythematosus (SLE), rheumatoid arthritis, and systemic sclerosis. ATG5 is also involved in both cancer suppression and progression. This description may include information annotated by UniProt and/or Google AI.