Product Name:
ATG4D
Product Number:
ab-nn211-2
Target Full Name: Autophagy-related cysteine endopeptidase 4
Target Alias: Autophagin-4; APG4D; ATG4D; Cysteine protease ATG4D; AUTL4; EC:3.4.22.-; AUT-like 4 cysteine endopeptidase; Autophagy-related protein 4 homologue D;
Product Type Specific: Autophagy protein pan-specific antibody
Antibody Code: NN211-2
Antibody Target Type: Pan-specific
Protein UniProt: Q86TL0
Protein SigNET: Q86TL0
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide from the N-terminal of Human ATG4D (AA 98-108)
Antibody Modification: Autophagy protein pan-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a medium intensity ~53 kDa protein band in HeLa cell lysates.
Antibody Species Reactivity: Human
Antibody Positive Control: A 1:1000 dilution of SPC-635 was sufficient for detection of ATG4D in 15 µg of Human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: No other prominent cross-reactive proteins in HeLa cell and mouse brain lysates.
Related Product 1: ATG4D pan-specific antibody (Cat. No.: AB-NN211-1)
Scientific Background: ATG4D, Auotphagy related 4D, cysteine peptidases, belong to the autophagy-related protein 4 family of C54 endopeptidases. Members of this family play a role in the biogenesis of autophagosomes (1). Cysteine protease ATG4D: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms (2).