Product Name:
ATG4C
Product Number:
ab-nn210-2
Target Full Name: AUT like 1cysteine endopeptidase (S. cerevisiae)
Target Alias: APG4 autophagy 4 homologue C; Cysteine protease ATG4C; APG4-C; Autophagy-related cysteine endopeptidase 3; EC:3.4.22.-; ATG 4C; AUT like 1; cysteine endopeptidase; EC 3.4.22; APG4C; Autophagin-3; OTTHUMP00000010715; AUTL3; APG4 autophagy 4 homologue C (S. cerevisiae); Autophagy related cysteine endopeptidase 3; ATG4C; Autophagin 3; ATG4 autophagy related 4 homologue C; AUT like 3 cysteine endopeptidase; AUTL1; ATG4C_HUMAN; AUT (S. cerevisiae) like 1; cysteine endopeptidase; Autophagy-related protein 4 homologue C; Autophagy related protein 4 homologue C; ATG4 autophagy related 4 homologue C (S. cerevisiae); Autophagy related 4C cysteine peptidase; Atg4c; APG4 C; FLJ14867; AUT-like 3 cysteine endopeptidase;
Product Type Specific: Autophagy protein pan-specific antibody
Antibody Code: NN210-2
Antibody Target Type: Pan-specific
Protein UniProt: Q96DT6
Protein SigNET: ATG4C
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide patterned after C-terminus of human ATG4C
Antibody Immunogen Sequence: RLGGERTNTDY
Antibody Immunogen Description: Corresponds to amino acid residues R296-Y306.
Production Method: Peptide Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a medium intensity ~53 kDa protein band in HEK-293T cell lysates.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Dog | Mouse.
Antibody Positive Control: A 1:1000 dilution of SPC-633 was sufficient for detection of ATG4C in 15 µg of Human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: No other prominent cross-reactive proteins in HEK-293T cell lysates.
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Scientific Background: ATG4C (Cysteine protease ATG4C) is a cysteine protease of the peptidase C54 family. It is required for the cytoplasm to vacuole transport (Cvt) and for autophagy. It is not essential for autophagy development under normal conditions but is required for a proper autophagic response under stressful conditions such as prolonged starvation. It cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. It also exhibits an activity of delipidating enzyme for the PE-conjugated forms. It is highly expressed in skeletal muscle, heart, liver and testis. This description may include information annotated by UniProt and/or Google AI.