Product Name:
ATG4C
Product Number:
ab-nn210-1
Target Full Name: Cysteine protease ATG4C
Target Alias: APG4 autophagy 4 homologue C; APG4 C; Atg4c; AUTL1; Autophagin 3
Product Type Specific: Autophagy protein pan-specific antibody
Antibody Code: NN210-1
Antibody Target Type: Pan-specific
Protein UniProt: Q96DT6
Protein SigNET: ATG4C
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: Synthetic peptide patterned after N-terminal of human ATG4C
Production Method: Peptide Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Predicted molecular mass at ~52.5 kDa. Observed molecular masses at ~45-60 kDa.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rat .
Antibody Specificity: High
Antibody Cross Reactivity: Two minor cross-reactive proteins in mouse brain lysates.
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Scientific Background: ATG4C (Cysteine protease ATG4C) is a cysteine protease of the peptidase C54 family. It is required for the cytoplasm to vacuole transport (Cvt) and for autophagy. It is not essential for autophagy development under normal conditions but is required for a proper autophagic response under stressful conditions such as prolonged starvation. It cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. It also exhibits an activity of delipidating enzyme for the PE-conjugated forms. It is highly expressed in skeletal muscle, heart, liver and testis. This description may include information annotated by UniProt and/or Google AI.