Product Name:
ATF4
Product Number:
ab-nn207-1
Target Full Name: Cyclic-AMP-dependent transcription factor ATF-4
Target Alias: Activating Transcription Factor 4; ATF 4; ATF4 protein; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; CREB 2; CREB-2; CREB2; Cyclic AMP dependent transcription factor ATF 4; Cyclic AMP response element binding protein 2; Cyclic AMP response element binding protein 2; Cyclic AMP-responsive element-binding protein 2; DNA binding protein TAXREB67; DNA binding protein TAXREB67; DNA-binding protein TAXREB67; Tax Responsive Enhancer Element B67; Tax-responsive enhancer element-binding protein 67; TAXREB67; TXREB
Product Type Specific: Transcription factor pan-specific antibody
Antibody Code: NN207-1
Antibody Target Type: Pan-specific
Protein UniProt: P18848
Protein SigNET: ATF4
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG2a
Antibody Immunogen Source: Fusion protein with human ATF4 peptide
Antibody Immunogen Description: Corresponds to amino acid residues L25-L327. 86% identical to rat, and 85% identical to mouse. <50% identity with ATF5.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~60 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Rat
Antibody Positive Control: 1 µg/ml of SMC-447 was sufficient for detection of ATF4 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Cross Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rat.
Scientific Background: ATF4 (Cyclic AMP-dependent transcription factor ATF-4) is a basic leucine-zipper (bZip) transcription factor, which regulates amino acid metabolism, DNA damage repair, chromatin remodeling, and apoptosis in response to cellular and ER stress. ATF4 works with various proteins, such as C/EBP homology protein (CHOP), aspargine synthetase (ASNS), and cAMP response element (CRE) among others to mediate cellular stress. ATF4 also regulates glucose homeostasis by suppressing beta-cell proliferation and insulin production. Furthermore, ATF4 targets the histone demethylase JMJD3 to alter chromatin structure and enhance gene transcription in response to amino acid deprivation. Under stress, its translation is preferential following the inactivation of initiation factor eIF2, allowing it to increase in concentration and regulate gene expression, often forming dimers with CHOP or CREB1. It features an bZIP domain for DNA binding/heterodimerization, a leucine zipper II (LZII) domain, and a PCAF domain. ATF4 acts as a pro-survival factor in specialized cells (e.g., osteoblasts, plasma cells) but can be pro-apoptotic (e.g., in neurons). It is involved in neurodegenerative diseases, tumour survival, and metabolic disorders (e.g., diabetes).Its activation is often found in cancer, promoting cell survival in hypoxic or nutrient-deprived tumors. This description may include information annotated by UniProt and/or Google AI.