Product Name:
Ankyrin-G
Product Number:
ab-nn197-1
Target Full Name: Ankyrin-3
Target Alias: ANK-3; ANK3; Ankryin G119; brain specific ankyrin G; CHANK3; ankyrin 3 (G); Ankyrin-3; Ankyrin-G; node of Ranvier (ankyrin G); Ankyrin 3; node of Ranvier; Ankyrin G119; FLJ45464; OTTHUMP00000217458; OTTHUMP00000217575; RP11 369L1.1
Product Type Specific: Cytoskeletal protein pan-specific antibody
Antibody Code: NN197-1
Antibody Target Type: Pan-specific
Protein UniProt: Q12955
Protein SigNET: Q12955
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein 1000 C-terminal amino acids of human Ankyrin G encompassing all of Ankyrin G with the exception of Ankyrin repeats
Antibody Modification: Cytoskeletal protein pan-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a >~200 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human | Mouse | Rat
Antibody Positive Control: 1 µg/ml of SMC-404 was sufficient for detection of Ankyrin-G in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Related Product 1: Ankyrin (pan) pan-specific antibody (Cat. No.: AB-NN195-2)
Related Product 2: Ankyrin-B pan-specific antibody (Cat. No.: AB-NN196-1)
Related Product 3: Ankyrin-B pan-specific antibody (Cat. No.: AB-NN196-2)
Related Product 4: Ankyrin-G pan-specific antibody (Cat. No.: AB-NN197-1)
Scientific Background: Ankyrins are a family of adaptor proteins that mediate the attachment of integral membrane proteins to the spectrin-actin based membrane skeleton (1). Ankyrins have binding sites for the beta subunit of spectrin and at least 12 families of integral membrane proteins. This linkage is required to maintain the integrity of the plasma membranes and to anchor specific ion channels, ion exchangers and ion transporters in the plasma membrane.