Product Name:
Ankyrin-B
Product Number:
ab-nn196-2
Target Full Name: Ankyrin-2
Target Alias: ANK-2; ANK2; Ankyrin 2; Ankyrin nonerythroid; Brain ankyrin; non-erythroid ankyrin; Ankyrin-B
Product Type Specific: Cytoskeletal protein pan-specific antibody
Antibody Code: NN196-2
Antibody Target Type: Pan-specific
Protein UniProt: Q01484
Protein SigNET: Ankyrin-B
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG2A
Antibody Immunogen Source: Synthetic peptide patterned after human Ankyrin-B
Antibody Immunogen Description: Corresponds to amino acid residues D203-E496.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a >200 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse.
Antibody Positive Control: 1 µg/ml of SMC-414 was sufficient for detection of Ankyrin-B in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Cross Reactivity: No cross-reactivity against Ankyrin-G.
Related Product 1: Ankyrin (pan) pan-specific antibody (Cat. No.: AB-NN195-2)
Related Product 2: Ankyrin R pan-specific antibody (Cat. No.: AB-NN195-1)
Related Product 3: Ankyrin-B pan-specific antibody (Cat. No.: AB-NN196-1)
Related Product 4: Ankyrin-G pan-specific antibody (Cat. No.: AB-NN197-1)
Scientific Background: Ankyrins are a family of adaptor proteins that mediate the attachment of integral membrane proteins to the spectrin-actin based membrane skeleton (1). Ankyrins have binding sites for the beta subunit of spectrin and at least 12 families of integral membrane proteins. This linkage is required to maintain the integrity of the plasma membranes and to anchor specific ion channels, ion exchangers and ion transporters in the plasma membrane. Ankyrin-B (ANK2) is a scaffolding protein that anchors membrane proteins to the spectrin cytoskeleton, acting as a crucial regulator of cellular organization, particularly in the heart and brain. It stabilizes ion channels and transporters (e.g., Na+/K+) ATPase, NCX1) in cardiomyocytes to maintain normal heart rhythm. For cardiac function, Ankyrin B acts as a master organizer for ion transporters (Na+/Ca2+ exchange type 1, Na+/K+ ATPase) and calcium signaling channels (IP3 receptors) in the heart, particularly in T-tubules and the sinoatrial node. In the brain, Ankyrin B plays a role in axonal cargo transport and restricts ankyrin-G positioning at the axon initial segment. It features 24 ANK repeats, ZU5 domains, a UPA domain, and Death domains (1 and 2), which are essential for its binding functionality. Loss-of-function Ankyrin variants, known as Ankyrin-B syndrome, cause cardiac arrhythmia (long QT syndrome type 4), sinus node dysfunction, and are associated with autism and epilepsy. This description may include information annotated by UniProt and/or Google AI.