Product Name:
Ankyrin (pan)
Product Number:
ab-nn195-2
Target Full Name: Ankyrin-1
Target Alias: Ankyrin-R; Erythrocyte Ankyrin; ANK-1; ANK; rCG_43073
Product Type Specific: Cytoskeletal protein pan-specific antibody
Antibody Code: NN195-2
Antibody Target Type: Pan-specific
Protein UniProt: P16157
Protein SigNET: Ankyrin (pan)
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein with full-length human Ankyrin-R
Antibody Immunogen Description: Corresponds to amino acid residues 1-1881 .
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: In mouse brain lysates, this antibody detects a ~200 kDa protein by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse.
Antibody Positive Control: A 1:100 dilution of SMC-488 was sufficient for detection of Ankyrin (pan) in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: Cross-reacts with Ankyrin-B and Ankyrin-G.
Related Product 1: Ankyrin R pan-specific antibody (Cat. No.: AB-NN195-1)
Related Product 2: Ankyrin-B pan-specific antibody (Cat. No.: AB-NN196-1)
Related Product 3: Ankyrin-B pan-specific antibody (Cat. No.: AB-NN196-2)
Related Product 4: Ankyrin-G pan-specific antibody (Cat. No.: AB-NN197-1)
Scientific Background: Ankyrins are a family of adaptor proteins that mediate the attachment of integral membrane proteins to the spectrin-actin based membrane skeleton (1). Ankyrins have binding sites for the beta subunit of spectrin and at least 12 families of integral membrane proteins. This linkage is required to maintain the integrity of the plasma membranes and to anchor specific ion channels, ion exchangers and ion transporters in the plasma membrane. Ankyrin R, or Ank1, was first discovered in erythrocytes, but has since also been located in the brain and muscles. Ankyrins (Ank1/RBC, Ank2/Brain, Ank3/General) serve as platforms for organizing protein networks, particularly in nerve cells (axon initial segment) and muscle cells. Ankyrin R (Ankyrin 1; Ank1) is a scaffolding protein that acts as a vital adapter, linking membrane proteins to the spectrin-actin cytoskeleton, establishing membrane domain structure. Ankyrin-R is primarily known for stabilizing erythrocyte membranes against shear stress, it also regulates fast-spiking neuronal activity, and it is crucial for node of Ranvier integrity. Ankyrin-R consists of three main functional domains: an N-terminal membrane-binding domain (24 ankyrin repeats), a spectrin-binding domain, and a C-terminal regulatory domain. It is highly expressed in the brain, particularly in cerebellum Purkinje neurons and GABAergic interneurons, where it supports the organization of ion channels (e.g., Kv3.1b) and perineuronal nets. It interacts with various proteins including spectrin, band 4.2, tubulin, and cell adhesion molecules. Mutations are associated with hereditary spherocytosis and Alzheimer's diease (2). This description may include information annotated by UniProt and/or Google AI.