Product Name:
AHA1
Product Number:
ab-nn190-2
Target Full Name: Activator of 90 kDa heat shock protein ATPase homologue 1
Target Alias: Aha 1; Ahsa1; p38; HSPC322; C14orf3; Activator of HSP90 ATPase
Product Type Specific: AHA1 pan-specific antibody
Antibody Code: NN190-2
Antibody Target Type: Pan-specific
Protein UniProt: O95433
Protein SigNET: AHA1
Antibody Type: Monoclonal
Antibody Host Species: Rat
Antibody Ig Isotype Clone: IgG2a Kappa
Antibody Immunogen Source: Mouse Aha1
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1.6 mg/ml
Storage Buffer: 0.02M potassium phosphate, 0.15M sodium chloride, pH7.2
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation | ELISA
Antibody Dilution Recommended: WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~38 kDa protein in cell and tissue lysates by Western blotting. Can migrate as a 45 kDa protein on SDS-PAGE gels based on immunoreactivity with recombinant Aha1.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse.
Antibody Positive Control: 1 µg/ml of SMC-173 was sufficient for detection of Aha1 in 10 µg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
Related Product 1: AHA1 pan-specific antibody (Cat. No.: AB-NN190-1)
Related Product 2: AHA1 pan-specific antibody (Cat. No.: AB-NN191-3)
Related Product 3: AHA1 pan-specific antibody (Cat. No.: AB-NN191-4)
Related Product 4: AHA2 pan-specific antibody (Cat. No.: AB-NN192-1)
Scientific Background: Aha1 is a 36-38 kDa member of the HSP90 cochaperone family, and is thought to stimulate HSP90 ATPase activity by competing with p23 and other co-chaperones for HSP90 binding (1, 2). This is crucial for the maturation of client proteins like kinases and steroid receptors. Through binding to the middle and N-terminal domains of Hsp90, Aha1 promotes a conformational change in Hsp90 to speed up its folding cycle. It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/HSP90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G) (3). Aha1 is involved in regulating protein quality control. It can promote the disposal of misfolded proteins by allowing ubiquitination of bound clients by the E3 ligase CHIP. Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors (4). This description may include information annotated by UniProt and/or Google AI.