Product Name:
PDK3-1
Product Number:
ab-nn181-1
Target Full Name: Pyruvate dehydrogenase kinase isoform 3; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3, mitochondrial
Target Alias: Kinase Pyruvate dehydrogenase kinase 3; PDK3
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NN181-1
Antibody Target Type: Pan-specific
Protein UniProt: Q15120
Protein SigNET: Q15120
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PDK3 (PDHK3) sequence peptide Cat. No.: PE-01BAC95
Antibody Immunogen Sequence: MRLFRWLLKQPVPKQC
Antibody Immunogen Description: Corresponds to amino acid residues M1 to Q15; N-terminus
Production Method: Corresponds to amino acid residues M1 to Q15; N-terminus
Antibody Modification: Protein kinase pan-specific antibody
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 1 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity with recombinant human PDK3 on protein dot blots.
Antibody Species Reactivity: Human; Mouse
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 42-53 kDa.
Antibody Specificity: Medium
Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human PDK1, PDK3 and PDK4.
Related Product 1: PDK3-1 blocking peptide
Related Product 2: PDK3-3 pan-specific antibody (Cat. No.: AB-NN181-2)
Related Product 3: PDHKSubtide - PDHK protein kinase substrate peptide
Scientific Background: PDHK3 (PDK3) is a protein-serine/threonine kinase of the Atypical group and PDHK family. It phosphorylates and inactivates the enzyme pyruvate dehydrogenase. It is mostly expressed in the heart and skeletal muscles, and it regulates glucose metabolism and maintenance of glucose homeostasis under starvation. Inactivation of PDHK3 signalling drives melanomas towards oxidative metabolism and allows for the possibility of using pro-oxidants as therapies. PDHK3 has been shown to be overexpressed in colon cancer cell lines.
