Product Name:
PDK1-2
Product Number:
ab-nn179-1
Target Full Name: Pyruvate dehydrogenase kinase isoform 1; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial
Target Alias: Kinase Pyruvate dehydrogenase kinase 1; Pyruvate dehydrogenase [lipoamide] kinase isozyme 1, mitochondrial precursor; Pyruvate dehydrogenase kinase isoform 1; Pyruvate dehydrogenase kinase, isozyme 1; PDK1
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NN179-1
Antibody Target Type: Pan-specific
Protein UniProt: Q15118
Protein SigNET: Q15118
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PDK1 (PDHK1) sequence peptide Cat. No.: PE-01AZX90
Antibody Immunogen Sequence: CNVLEVIKDGYENARR
Antibody Immunogen Description: Corresponds to amino acid residues C223 to R238
Production Method: Corresponds to amino acid residues C223 to R238
Antibody Modification: Protein kinase pan-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 1 µg/ml for immunoblotting
Antibody Potency: Very strong immunoreactivity with recombinant human PDK1 on protein dot blots.
Antibody Species Reactivity: Human; Mouse
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 45-65 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human PDK2, PDK3 and PDK4.
Related Product 1: PDK1-2 blocking peptide
Related Product 2: PDK1-3 pan-specific antibody (Cat. No.: AB-NN179-2)
Related Product 3: PDHKSubtide - PDHK protein kinase substrate peptide
Scientific Background: PDHK1 (PDK1, pyruvate dehydrogenase kinase 1) is a protein-serine/threonine kinase of the Atypical group and PDHK family. It functions in the regulation of glucose and fatty acid metabolism through the phosphorylation of the subunits PDHA1 and PDHA2 of pyruvate dehydrogenase. This inhibitory phosphorylation reduces metabolite flux into the trichloroacetic acid (TCA) cycle, decreases aerobic respiration, and prevents the formation of acetyl-CoA. In addition, PDHK1 functions to prevent apoptosis in cells exposed to hypoxia or oxidative stress. Due to the presence of HIF-1, PDHK1 has shown to have increased activity in hypoxic cancer cells. PDHK1 shunts pyruvate away from the citric acid cycle and helps to maintain hypoxic cell viability. PDHK1 may be a target for antitumour therapy, since PDK1 prevents apoptosis in these cancerous cells.
