Product Name:
Calreticulin
Product Number:
ab-nn137-1
Target Full Name: Calreticulin
Target Alias: CALR; Calregulin; cC1qR; CRP55; ERp60; HSCBP; RO; SSA; grp60
Product Type Specific: Calcium binding protein pan-specific antibody
Antibody Code: NN137-1
Antibody Target Type: Pan-specific
Protein UniProt: P27797
Protein SigNET: P27797
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human calreticulin synthetic peptide with a cysteine residue added and the peptide conjugated to KLH
Antibody Modification: Calcium binding protein pan-specific antibody
Storage Buffer: Rabbit antiserum
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting, Immunohistochemistry, ICC/Immunofluorescence, Immunoprecipitation
Antibody Dilution Recommended: 1:5000-10000 (ECL) (WB)
Antibody Potency: Very high potency. Detects a ~63 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: Human, Mouse, Rat, Bovine, Canine, Chicken, Drosophila, Fish (carp), Guinea pig, Hamster, Monkey, Pig, Rabbit, Sheep
Antibody Positive Control: A 1:5000 dilution of SPC-122 was sufficient for detection of Calreticulin in 20μg of HeLa cell lysate by ECL immunoblot analysis.
Antibody Specificity: Very high
Scientific Background: Calreticulin is a multifunctional, highly conserved Ca2+ -binding protein that is localized to the endoplasmic reticulum (ER), but has also been detected in the nucleus and nuclear envelop. Like many other ER proteins, it has the conserved ER retention KDEL (Lys-Asp-Glu-Leu) sequence at its C-terminus (1-3). CRT’s three domains include a 180 residue N-terminal domain, a proline-rich P-domain (residues 189-288) that binds Ca2+ with high affinity and shares homology with calnexin (CNX) and calmegin, and a 110 residue C-terminal domain that binds Ca2+ with low affinity but high capacity (1,3). Recent studies suggest that this soluble ER protein has a multifunctional role. It appears to be involved in calcium storage and regulation as well as having a molecular chaperone activity. It has been shown to interact with the cytoskeleton and to be involved in the regulation of gene expression. Calreticulin may also play a role in cellular proliferation including its apparent activity in the proliferation of certain viruses within mammalian host cells (4, 5), and it has also been shown to be induced in response to various types of cell stress including amino acid deprivation (6). Close interconnections among protein synthesis, gene expression and calcium signaling have been observed by many researchers in recent years. Calreticulin might be centrally located and therefore it crucially participates in the coordination of many functions by the cell (4, 5). Studies also suggest its involvement in a few diseases such as systemic lupus erythematosus, rheumatoid arthritis, celiac disease, complete congenital heart block, and halothane hepatitis (1).
