Product Name:
FKBP52
Product Number:
ab-nn127
Target Full Name: Peptidyl-prolyl cis-trans isomerase FKBP4
Target Alias: FK506 binding protein 4; FKBP4; FKBP59; HBI; HSP56; p52; p59; PPIase; Rotamase; T cell FK506 binding protein
Product Type Specific: FK506 binding protein pan-specific antibody
Antibody Code: NN127
Antibody Target Type: Pan-specific
Protein UniProt: Q02790
Protein SigNET: Q02790
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG
Antibody Immunogen Source: Synthetic peptide corresponding to the residues of human FKBP52
Antibody Modification: FK506 binding protein pan-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation
Antibody Dilution Recommended: WB (1:2000), IHC (1:250), IP (5µg); optimal dilutions for assays should be determined by the user.
Antibody Potency: Medium potency. Detects a ~52 kDa protein in cell and tissue lysates by Western blotting. Heavy chain migrates close to FKBP52 on SDS-PAGE gels.
Antibody Species Reactivity: Human | Mouse | Rat | Dog | Hamster
Antibody Positive Control: 0.5 µg/ml was sufficient for detection of FKBP52 in 20 µg total protein using WB by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
Antibody Specificity: Very high
Related Product 1: FKBP51 pan-specific antibody (Cat. No.: AB-NN249-1)
Scientific Background: HSP90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones (1). One class of HSP90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an HSP90 binding site. Among the TPR cochaperones of HSP90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin A-binding families of immunophilins (2). FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex (3). The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the HSP90 (4). Although FKBP52 and FKBP51 share ~75% sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different HSP90-binding characteristics (3). FK506 binding protein 51 kDa (FKBP51 or otherwise referred to as FKBP54) has been identified as a progestininducible gene. This protein is predominantly expressed in murine T cells but in humans, it is abundantly expressed in numerous tissues at levels many times higher than FKBP12. The FKBP51 gene is known to be induced by glucocorticoids (5).
