Product Name:
SOD1 (UβB)
Product Number:
ab-nn098-5
Target Full Name: Superoxide dismutase [Cu-Zn]
Target Alias: Superoxide dismutase 1; SOD; SOD1 unfolded beta barrel region
Product Type Specific: SOD1 (Cu-Zn) superoxide dismutase pan-specific antibody
Antibody Code: NN098-5
Antibody Target Type: Pan-specific
Protein UniProt: P00441
Protein SigNET: SOD1 (UβB)
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Ig Isotype Clone: N/A
Antibody Immunogen Source: N-terminal region of SOD1, unfolded beta barrel region
Production Method: Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: Recognizes a conformation specific epitope where the beta barrel is unfolded.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse.
Antibody Positive Control: 1µg/ml of SPC-205 was sufficient for detection of the unfolded beta barrel of SOD1 by colorimetric dot blot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Related Product 1: SOD (Cu/Zn) expression antibody (Cat. No.: AB-NN098-2)
Related Product 2: SOD (Cu/Zn) expression antibody (Cat. No.: AB-NN098-3)
Related Product 3: SOD1 S108 phosphorylation antibody (Cat. No.: AB-PN903)
Related Product 4: SOD1 S99 phosphorylation antibody (Cat. No.: AB-PN904)
Related Product 5: SOD1 T40 phosphorylation antibody (Cat. No.: AB-PN905)
Related Product 6: SOD1 (EDI) expression antibody (Cat. No.: AB-NN098-4)
Related Product 7: SOD (EC) expression antibody (Cat. No.: AB-NN328-1)
Related Product 8: SOD (EC) expression antibody (Cat. No.: AB-NN328-2)
Related Product 9: SOD (Mn) expression antibody (Cat. No.: AB-NN068-1)
Related Product 10: SOD (Mn) expression antibody (Cat. No.: AB-NN068-2)
Scientific Background: SOD1 (cytoplasmic copper-zinc superoxide dismutase) serves as an important antioxidant that catalyzes the conversion of the toxic reactive oxygen species superoxide radical O2ˉ anion into oxygen (O2) and hydrogen peroxide (H2O2), which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (1,4). It is an intracellular metalloenzyme that is found in almost every cell in the body (2). In general, SODs play a major role in antioxidant defense mechanisms (3). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (2). Misfolding of SOD1 has been implicated in amyotrophic lateral sclerosis (ALS). Over 180 different mutations in SOD1 have been linked to ALS. Conformation specific antibodies such as SOD1 (EDI), which targets an exposed region of the dimer interface (EDI) of SOD1, are useful for determining the conformation of SOD1 in affected tissues (5). This antibody can be used in conjunction with SOD1 (UβB) (SPC-205D) which detects an unfolded beta barrel (UβB) of SOD1 This description may include information annotated by UniProt and/or Google AI.