Product Name:
PCNA
Product Number:
ab-nn087-2
Target Full Name: DNA polymerase delta auxiliary protein
Target Alias: cb16; MGC8367; PCNA_HUMAN; Polymerase delta accessory protein; Proliferating cell nuclear antigen; Mutagen-sensitive 209 protein; OTTHUMP00000030189; PCNAR; Pcna/cyclin; HGCN8729; wu:fb36g03; PCNA; ATLD2; fb36g03; OTTHUMP00000030190; Cyclin
Product Type Specific: DNA synthesis protein pan-specific antibody
Antibody Code: NN087-2
Antibody Target Type: Pan-specific
Protein UniProt: P12004
Protein SigNET: PCNA
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide of Human PCNA
Production Method: Peptide Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a weak ~37 kDa protein band in human 293 Trap cell lysates.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human.
Antibody Positive Control: A 1:1000 dilution of SPC-691 was sufficient for detection of PCNA in 15 µg of Human 293T cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: No other prominent cross-reactive proteins in mouse brain and 293 Trap cell lysates.
Scientific Background: PCNA (DNA sliding clamp proliferating cell nuclear antigen) is an auxiliary 29 kDa homotrimeric, ring-shaped protein that clamps around double-stranded DNA, allowing it to slide along the DNA. It is a component of the DNA polymerase delta and epsilon complexes, and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. It induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. It functions in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. It acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion. It is primarily localized in the nucleus, with its expression is highest during the S phase of the cell cycle, making it a common marker for proliferation. It is controlled by E2F transcription factors and subjected to post-translational modifications (ubiquitination, SUMOylation). This description may include information annotated by UniProt and/or Google AI.