Product Name:
p53
Product Number:
ab-nn082-2
Target Full Name: Tumour suppressor protein p53 (antigen NY-CO-13)
Target Alias: P53; LFS1; TRP53; FLJ92943; TP53; p53 Tumour Suppressor; Phosphoprotein p53; TP53; Transformation related protein 53; TRP53; Tumour protein p53
Product Type Specific: TP53 transcription protein pan-specific antibody
Antibody Code: NN082-2
Antibody Target Type: Pan-specific
Protein UniProt: P04637
Protein SigNET: p53
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein for human p53
Antibody Immunogen Sequence: SPDDIEQW
Antibody Immunogen Description: Corresponds to amino acid residues S46-W53.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | Immunoprecipitation | ELISA | RIA | FCM
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~53 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque .
Antibody Positive Control: 1 µg/ml of SMC-183 was sufficient for detection of p53 in 10 µg of HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Related Product 1: p53 expression antibody (Cat. No.: AB-NN082-3)
Related Product 2: p53 S6+S9 phosphorylation antibody (Cat. No.: AB-PN637)
Related Product 3: p53 T18+S20 phosphorylation antibody (Cat. No.: AB-PN638)
Related Product 4: p53 S392 phosphorylation antibody (Cat. No.: AB-PN640)
Related Product 5: p53 S15 phosphorylation antibody (Cat. No.: AB-PN859)
Scientific Background: The p53 protein ( tumour protein 53 or TP53) is a DNA-binding cell cycle-regulating transcription factor that governs cell division and the fine balance between cell death and cell survival (1). P53 plays a critical role in tumour suppression and hence it is often described as "the guardian of the genome", "the guardian angel gene", or the "master watchman." This also refers to its role in conserving stability by preventing genome mutation (2). Defects in p53 are linked to >50% of human cancers, and restoring p53 function to these cancer cells can induce growth arrest and apoptosis (3). When p53 has been damaged, it can also lead to autoimmune disorders (4, 5). This description may include information annotated by UniProt and/or Google AI.