Product Name:
HSP47
Product Number:
ab-nn058-2
Target Full Name: Serpin H1
Target Alias: Colligin; Gp46; serine proteinase inhibitor; cysteine proteinase inhibitor; collagen binding protein
Product Type Specific: HSP47 heat shock/stress protein pan-specific antibody
Antibody Code: NN058-2
Antibody Target Type: Pan-specific
Protein UniProt: P50454.2
Protein SigNET: HSP47
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1Kappa
Antibody Immunogen Source: Recombinant full length human HSP47
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.4, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~47 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human.
Antibody Positive Control: 1 µg/ml of SMC-203 was sufficient for detection of HSP47 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti -mouse IgG:HRP as the secondary antibody.
Scientific Background: HSP47 is a chaperone protein, member of the superfamily of serine proteinase inhibitors. It is also known as SERPINH1, a serine proteinase inhibitor. It is a stress protein that resides in the endoplasmic reticulum, has an active role on the intracellular process of folding, assembly and secretion of pro-collagens. It stabilizes newly synthesized procollagen and it prevents the aggregation of misfolded collagen in the ER. It also facilitates the transport of procollagen from the ER to the Golgi apparatus. Elevated levels of HSP47 are strongly associated with increased collagen deposition in fibrotic diseases (liver, lung) (1). It is highly inducible by heat shock and stress, but also expressed constitutively in tissues that actively produce collagen. It is identified as a novel biomarker on cell therapies aimed to reduce the progression of fibrotic diseases, and ould be used potentially as a universal marker, since HSP47 binds a single substrate (2). Type I collagen is fundamental during the healing process after a myocardial infarction. It is critical in the position of collagen-produced cells and the assembly of collagen fibrils (3). Deficiency or mutations in HSP47 can cause severe, sometimes lethal, collagen folding diseases like Osteogenesis Imperfecta. This description may include information annotated by UniProt and/or Google AI.