Product Name:
HSP40, YDJ1
Product Number:
ab-nn057-5
Target Full Name: Mitochondrial protein import protein MAS5
Target Alias: DNAJA2; CPR3; HIRIP4; DNAJ; DNJ3; DJ3; RDJ2; HIRa interacting protein4
Product Type Specific: Heat shock/stress protein pan-specific antibody
Antibody Code: NN057-5
Antibody Target Type: Pan-specific
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1 Kappa
Antibody Immunogen Source: Full length protein yeast HSP40 (YDJ1)
Antibody Modification: Heat shock/stress protein pan-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunoprecipitation | ELISA
Antibody Dilution Recommended: WB (1:2000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~40 kDa protein by Western blotting. Yeast specific product.
Antibody Species Reactivity: Yeast
Antibody Positive Control: 0.5 µg/ml of SMC-150 was sufficient for detection of 50ng YDJ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: Does not cross react with human, mouse or rat HSP40.
Related Product 1: HSP40 pan-specific antibody (Cat. No.: AB-NN057-3)
Related Product 2: HSP40 pan-specific antibody (Cat. No.: AB-NN057-4)
Related Product 3: HSP40, Hdj1 pan-specific antibody (Cat. No.: AB-NN057-2)
Related Product 4: HSP40, YDJ1 pan-specific antibody (Cat. No.: AB-NN057-6)
Scientific Background: Human HSP40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). There are two main types of HSP40; type 1 DNAJ proteins including HDJ2 and yeast YdjI; type II includes yeast Sis1 and human Hdj1. Whereas type I possesses a zinc finger domain which helps in the function of protein folding. (3, 4), type II does not. Members of the HSP40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins (5, 6). The HSP40-HSP70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (7).