Product Name:
HSP40, YDJ1
Product Number:
ab-nn057-5
Target Full Name: Mitochondrial protein import protein MAS5
Target Alias: DNAJA2; CPR3; HIRIP4; DNAJ; DNJ3; DJ3; RDJ2; HIRa interacting protein4
Product Type Specific: Heat shock/stress protein pan-specific antibody
Antibody Code: NN057-5
Antibody Target Type: Pan-specific
Protein UniProt: P25491
Protein SigNET: HSP40, YDJ1
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1 Kappa
Antibody Immunogen Source: Full length protein yeast HSP40 (YDJ1) (Uniprot ID P25491)
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunoprecipitation | ELISA
Antibody Dilution Recommended: WB (1:2000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~40 kDa protein by Western blotting. Yeast specific product.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Yeast.
Antibody Positive Control: 0.5 µg/ml of SMC-150 was sufficient for detection of 50ng YDJ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: Does not cross react with human, mouse or rat HSP40.
Related Product 1: HSP40, YDJ1 expression antibody (Cat. No.: AB-NN057-6)
Related Product 2: HSP40, Hdj1 expression antibody (Cat. No.: AB-NN057-2)
Related Product 3: HSP40 expression antibody (Cat. No.: AB-NN057-3)
Related Product 4: HSP40 expression antibody (Cat. No.: AB-NN057-4)
Scientific Background: Human HSP40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). Members of the HSP40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. In particular, Hdj1, the first human HSP40/DnaJ protein identified, plays an important role in protein translation and folding, as well as in the regulation of HSP70 function (3). HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins (4, 5). The HSP40-HSP70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (6). Some studies have shown that the difference between HDJ1 and type 1 DNAJ proteins including HDJ2 and yeast YdjI is the result of the possession of a zinc finger domain by the latter, which helps in the function of protein folding. (7, 8). HSP40 is involved in protein trafficking, autophagy, and signal transduction, with roles in neurodegenerative diseases and cancer. This description may include information annotated by UniProt and/or Google AI.