Product Name:
HSP40, Hdj1
Product Number:
ab-nn057-2
Target Full Name: DnaJ homolog subfamily B member 1
Target Alias: DNAJ1; NDAJB1; HDJ1; HSP40; HSPF1; DnaJ homologue subfamily B member 1; Dna J protein homologue 1; Heat shock 40 kDa protein 1; HSP40; heat shock protein 40; Human DnaJ protein 1; hDj-1
Product Type Specific: Heat shock/stress protein pan-specific antibody
Antibody Code: NN057-2
Antibody Target Type: Pan-specific
Protein UniProt: P25685
Protein SigNET: HSP40, Hdj1
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Recombinant protein HSP40 (Hdj1)
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH7.2, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence | Immunoprecipitation | ELISA
Antibody Dilution Recommended: WB (1:2000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Antibody Potency: High potency. Detects a ~40 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse.
Antibody Positive Control: 0.5 µg/ml of SMC-145 was sufficient for detection of HSP40 (HDJ1) in 15 µg of HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: Does not cross-react with HDJ2 or YDJ1.
Related Product 1: HSP40, YDJ1 expression antibody (Cat. No.: AB-NN057-5)
Related Product 2: HSP40, YDJ1 expression antibody (Cat. No.: AB-NN057-6)
Related Product 3: HSP40 expression antibody (Cat. No.: AB-NN057-3)
Related Product 4: HSP40 expression antibody (Cat. No.: AB-NN057-4)
Scientific Background: DnaJ/HSP40 proteins have been preserved throughout evolution and are important for protein translation, folding, unfolding, translocation, and degradation, primarily by stimulating the ATPase activity of chaperone proteins, HSP70s. Because the ATP hydrolysis is essential for the activity of HSP70s, DnaJ/HSP40 proteins actually determine the activity of HSP70s by stabilizing their interaction with substrate proteins. DnaJ/HSP40 proteins all contain the J domain through which they bind to HSP70s. HSP40, also known as HDJ1 (1), is a basic mammalian 40kDa heat shock protein which is not only homologous to the bacterial heat shock protein (DnaJ), but also yeast DnaJ-related proteins such as SCJ1, Sec63/Npl1, YDJ1 and SIS1 (2-6). HSP 40 is inducible by stress including heat after which is moves from the cytoplasm to the nucleus and nucleoli; an intracellular pattern similar to HSC70/HSP70, the mammalian homologues of the bacterial heat shock protein, DnaK (3). PF11_0513 belongs to the HSP40 family of chaperones. This protein has a Plasmodium export element (PEXEL). It is exported out of the parasite into the infected erythrocytic compartment. This description may include information annotated by UniProt and/or Google AI.