Product Name:
14-3-3-Z-2
Product Number:
ab-nn001-3
Target Full Name: 14-3-3 protein zeta/delta
Target Alias: 14-3-3-zeta; 1433Z; 14-3-3-zeta; 143Z; Factor activating exoenzyme S; FAS; KCIP-1; Mitochondrial import stimulation factor S1 subunit; Protein kinase C inhibitor protein-1; Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide; YWHAZ
Product Type Specific: 14-3-3 protein zeta/delta pan specific antibody
Antibody Code: NN001-3
Antibody Target Type: Pan-specific
Protein UniProt: P63104
Protein SigNET: 14-3-3-Z
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide patterned after human 14
Antibody Immunogen Sequence: CAAGDDKKGIVDQSQQA
Antibody Immunogen Description: Corresponds to amino acid residues A133 to A148.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Platypus | Chicken |Frog | Zebra fish | Fruit fly | Honey bee | Nematode worm.
Related Product 1: 14-3-3 expression antibody (Cat. No.: AB-NN441-2)
Related Product 2: 14-3-3 expression antibody (Cat. No.: AB-NN441-3)
Scientific Background: 14-3-3 proteins are adapter/scaffold protein that bind serine or threonine phosphorylated proteins. 14-3-3 zeta adapter protein is implicated in the regulation of a large spectrum of both general and specialized signalling pathways. Its binding to target phosphoproteins generally results in the modulation of the activity of the binding partner. It induces ARHGEF7 activity on RAC1 as well as lamellipodia and membrane ruffle formation. In neurons, 14-3-3 zeta regulates spine maturation through the modulation of ARHGEF7 activity. This description may include information annotated by UniProt and/or Google AI.