Product Name:
ULK3
Product Number:
ab-nk355-1
Target Full Name: Unc-51 (C. elegan) -like kinase 3
Target Alias: Serine/threonine-protein kinase ULK3; EC:2.7.11.1; Ulk3; DKFZP434C131; FLJ90566; ULK3_HUMAN; unc 51 like kinase 3 (C. elegans); ULK3;
Product Type Specific: ULK3 protein kinase pan-specific antibody
Antibody Code: NK355-1
Antibody Target Type: Pan-specific
Protein UniProt: Q6PHR2
Protein SigNET: ULK3
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide from the N-terminal of Human ULK3
Antibody Immunogen Sequence: PWDEKHVLRGSP
Antibody Immunogen Description: Corresponds to amino acid residues P166-177P.
Production Method: Peptide Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a medium ~53 kDa protein band in A549 cell lysates and more weakly in rat and mouse brain lysates.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse.
Antibody Positive Control: A 1:1000 dilution of SPC-642 was sufficient for detection of ULK3 in 15 µg of Human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: High
Antibody Cross Reactivity: No other prominent cross-reactive proteins in mouse and rat brain lysates or A431 cell lysates except for a ~30 kDa cross-reactive protein.
Related Product 1: ULK1 pan-specific antibody (Cat. No.: AB-NK298-2)
Related Product 2: ULK2 pan-specific antibody (Cat. No.: AB-NK354-1)
Scientific Background: ULK3 (UNC-51 like kinase 3) is a ~58 kDa serine/threonine-protein kinase that regulates critical cellular processes, including Sonic hedgehog (SHH) signaling, autophagy, and cytokinetic abscission. It acts as a molecular switch, activating GLI transcription factors (GLI1, GLI2, GLI3) in the SHH pathway by promoting their nuclear translocation, and, when stressed, phosphorylates ESCRT-III complex components to control cell division timing. ULK3 controls the timing of cytokinetic abscission (the final separation of daughter cells) by binding to IST1 and phosphorylating ESCRT-III, particularly during cellular stress, such as lagging chromosomes or nuclear pore defects. It is involved in the regulation of autophagy, the cellular recycling process, particularly in response to senescence. It features an N-terminal kinase domain and a C-terminal regulatory domain. Its catalytic activity is regulated by phosphorylation in the kinase domain. Increased expression is associated with cancer-associated fibroblasts (CAFs) and tumor-enhancing properties, particularly in squamous cell carcinomas. This description may include information annotated by UniProt and/or Google AI.