Product Name:
ULK2
Product Number:
ab-nk354-1
Target Full Name: Unc 51 (C. elegans) like kinase 2
Target Alias: Serine/threonine-protein kinase ULK2; EC:2.7.11.1; ULK2_HUMAN; KIAA0623; ATG1B; Serine/threonine protein kinase ULK2; Unc-51-like kinase 2; Unc 51 like autophagy activating kinase 2; Unc51.2; ULK2; Unc 51 like kinase 2;
Product Type Specific: ULK2 protein kinase pan-specific antibody
Antibody Code: NK354-1
Antibody Target Type: Pan-specific
Protein UniProt: Q8IYT8
Protein SigNET: ULK2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide from the mid-region of human ULK2
Antibody Immunogen Sequence: GYSYSPQPSRPG
Antibody Immunogen Description: Corresponds to amino acid residues G558-G569.
Production Method: Peptide Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a strong ~113 kDa protein band in HeLa cell lysates.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rat.
Antibody Positive Control: A 1:1000 dilution of SPC-641 was sufficient for detection of ULK2 in 15 µg of Human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: No other prominent cross-reactive proteins in mouse brain and HeLa cell lysates.
Related Product 1: ULK1 pan-specific antibody (Cat. No.: AB-NK298-2)
Related Product 2: ULK3 pan-specific antibody (Cat. No.: AB-NK355-1)
Scientific Background: ULK2 (UNC-51 like kinase 2) is a serine/threonine protein kinasr that acts as a key regulator of autophagy in response to nutrient starvation, promoting cellular homeostasis. It works early in the autophagy pathway to initiate autophagophore formation and also plays a critical role in neuronal development, particularly in axon formation. It contains an amino-terminal kinase domain followed by a central proline-serine rich domain and a highly conserved carboxy-terminal domain. ULK2 serves as a downstream effector of AMPK and a regulator of mTORC1, often functioning alongside ULK1 in initiating autophagy. It also acts as a negative regulator of mTORC1 and AMPK signaling pathways. It has been linked to axon growth and is essential for autophagy. Structurally, ULK2 is similar to ATG1. It is highly expressed in the brain, playing a crucial role in endocytosis, axonal elongation, and neuronal differentiation. This description may include information annotated by UniProt and/or Google AI.