Product Name:
PINK1
Product Number:
ab-nk304-1
Target Full Name: Serine/threonine-protein kinase PINK1, mitochondrial (PTEN-induced putative kinase protein 1)
Target Alias: Parkinson disease (autosomal recessive) 6; EC 2.7.11.1; PARK6; BRPK; FLJ27236; PTEN induced putative kinase 1; Phosphatase and Tensin Homologue
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK304-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9BXM7
Protein SigNET: PINK1
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein with cytoplasmic C-terminus of human PINK1
Antibody Immunogen Description: Corresponds to amino acid residues E112-K496. 82% identical to rat and 81% identical to mouse. >30% identity with DMPK.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Immunohistochemistry | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a ~50 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse.
Antibody Positive Control: 1 µg/ml of SMC-450 was sufficient for detection of PINK1 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Related Product 1: PINK1 S228 phosphorylation antibody (Cat. No.: AB-PK906)
Related Product 2: PINK1 T313 phosphorylation antibody (Cat. No.: AB-PK907)
Related Product 3: PINK1 S402 phosphorylation antibody (Cat. No.: AB-PK908)
Related Product 4: PINK1 T257 phosphorylation antibody (Cat. No.: AB-PK942)
Scientific Background: PINK1 (PTEN induced putative kinase 1) is a mitochondrial serine/threonine kinase which maintains mitochondrial function/integrity, provides protection against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins, and is involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). PINK1 is synthesized as a 63 kDa protein which undergoes proteolyt processing to generate at least two cleaved forms (55 kDa and 42 kD). PINK1 and its substrates have been found in the cytosol as well as in different sub-mitochondrial compartments, and according to the recent reports; PINK1 may be targeted to OMM (outer mitochondrial membrane) with its kinase domain facing the cytosol, providing a possible explanation for the observed physical interaction with the cytosolic E3 ubiquitin ligase Parkin. It regulates axonal transport by phosphorylating mitochondrial Rho GTPase 1 (Miro1) (1). Defective PINK1 may cause alterations in processing, stability, localization and activity as well as binding to substrates/interaction-partners which ultimately leads to differential effects on mitochondrial function and morphology. Mutations in PINK1 are linked to autosomal recessive early onset Parkinson's disease, and are associated with loss of protective function, mitochondrial dysfunction, aggregation of alpha-synuclein, as well as proteasome dysfunction. Downregulation of PINK1 suppressed the TDP- 43-induced degenerative phenotypes in a Drosophila model, and PINK1 also functioned as a genetic modifier of FUS- induced neurodegeneration (2,3). This description may include information annotated by UniProt and/or Google AI.