Product Name:
LRRK2/Dardarin
Product Number:
ab-nk303-2
Target Full Name: Leucine-rich repeat serine/threonine-protein kinase 2
Target Alias: Leucine-rich repeat kinase 2; RIP7; PARK8; Dardarin; ROCO 2; RIPK7; augmented in rheumatoid arthritis 17
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK303-2
Antibody Target Type: Pan-specific
Protein UniProt: Q5S007
Protein SigNET: LRRK2/Dardarin
Antibody Type: Monoclonal
Antibody Host Species: Mouse
Antibody Ig Isotype Clone: IgG1
Antibody Immunogen Source: Fusion protein with human LRRK2 N-terminal half.
Antibody Immunogen Description: Corresponds to amino acid residues M1-V500. 83% identical in mouse and rat. No significant identity with LRRK1.
Production Method: Protein G purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 50% glycerol, 0.1% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | ICC/Immunofluorescence
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a >200 kDa protein in cell and tissue lysates by Western blotting.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rat | Mouse.
Antibody Positive Control: 1 µg/ml of SMC-446 was sufficient for detection of LRRK2/Dardarin in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Related Product 1: LRRKtide KinSub kinase substrate peptide (Cat. No.: PE-01ADD95)
Related Product 2: LRRKSubtide kinase substrate peptide (Cat. No.: PE-01BHX95)
Related Product 3: LRRK2/Dardarin expression antibody (Cat. No.: AB-NK303-1)
Related Product 4: LRRK2 S935 phosphorylation antibody (Cat. No.: AB-PK905)
Related Product 5: LRRK2 S955 phosphorylation antibody (Cat. No.: AB-PK935)
Related Product 6: LRRK2 S973 phosphorylation antibody (Cat. No.: AB-PK936)
Related Product 7: LRRK2 T1410 phosphorylation antibody (Cat. No.: AB-PK937)
Related Product 8: LRRK2 T1503 phosphorylation antibody (Cat. No.: AB-PK938)
Related Product 9: LRRK2 T2031+S2032 phosphorylation antibody (Cat. No.: AB-PK939)
Scientific Background: LRRK2 (Leucine-rich repeat kinase 2; Dardarin) is a 268 kDa protein with multiple domains including several ankyrin, leucine-rich, and WD40 repeats, a Ras-like small GTPase family domain named ROC, and a MAPKKK kinase domain that is closely related to the RIP kinase domain. It acts as a signaling molecule and scaffold protein, phosphorylating a subgroup of Rab GTPases (e.g., Rab8A, Rab10) to regulate intracellular trafficking and lysosomal functions. LRRK2 positively regulates autophagy through a calcium-dependent activation of the CaMKK/AMPK signalling pathway. The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increase in lysosomal pH, and calcium release from lysosomes. It is found in the cytoplasm and associated with membranes of organelles such as lysosomes, endosomes, and the Golgi apparatus. LRRK2 is expressed in brain as well as in other tissues such as lung, liver and heart. It is also highly expressed in immune cells, particularly neutrophils. LRRK2 might be central to the pathogenesis of several major neurodegenerative diseases associated with Parkinsonism. Mutations (e.g., G2019S, R1441G) typically cluster in the catalytic ROC-COR-Kinase core, increasing kinase activity. Increased kinase activity disrupts cellular trafficking and autophagic processes, leading to neurodegeneration. Several dominantly inherited missense mutations in the gene encoding LRRK2 have been identified in several families that exhibit a broad spectrum of neuropathological features. LRRK2 is also linked to Crohn's disease and Hansen’s disease. This description may include information annotated by UniProt and/or Google AI.