Product Name:
ZC2-CT
Product Number:
ab-nk301-1
Target Full Name: TRAF2 and NCK-interacting kinase
Target Alias: TNIK; KIAA0551; TRAF2 and NCK interacting kinase
Product Type Specific: TNIK protein kinase pan-specific antibody
Antibody Code: NK301-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9UKE5
Protein SigNET: ZC2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human ZC2 (TNIK) sequence peptide Cat. No.: PE-01BFN, PE-01BFO
Antibody Immunogen Sequence: CGSSQVFFMTLNRNSMMNW
Antibody Immunogen Description: Corresponds to amino acid residues G1343 to W1360; C-terminus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Mouse | Chicken.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 180-200 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactivities detected in various human cancer cell lines, including A431, HEK-293, HeLa, HepG2, Jurkat and MCF7 cells. No cross-reactivities detected in diverse mouse tissues, except for a weak ~50 kDa protein in mouse skeletal muscle and thymus.
Related Product 1: ZC2-CT 1343-2360) antibody blocking peptide (Cat. No.: PE-01BFN75)
Scientific Background: ZC2 (TNIK; TRAF2 and NCK-interacting protein kinase) is a protein-serine/threonine kinase of the STE group and STE20 family. It may be involved in cytoskeleton regulation. Mutations at the 54, 152-153 and 171-172 positions of ZC2 lead to loss of autophosphorylation. It acts as an essential activator of the Wnt signalling pathway, and is recruited to promoters of Wnt target genes and required to activate their expression. It may act by phosphorylating TCF4/TCF7L2, and appears to function upstream of the JUN N-terminal pathway. It may mediate part of the response to environmental stress. It is part of a signalling complex composed of NEDD4, RAP2A and TNIK, which regulates neuronal dendrite extension and arborization during development. In the brain, ZC2 regulates dendrite development and synaptic transmission. More generally, it may play a role in cytoskeletal rearrangements and regulate cell spreading. It phosphorylates SMAD1 on Thr-322. It features an N-terminal kinase domain, an intermediate domain, and a C-terminal Citron homology (CNH) domain, which mediates its interaction with TRAF2 and NCK. ZC2 deficiency can protect against obesity and insulin resistance. Mutations in the ZC2 gene have been linked to autosomal recessive forms of intellectual disability. ZC2 is highly expressed in various cancers, including colorectal, pancreatic, and lung cancer, where it drives cancer stem cell function and tumor growth. This description may include information annotated by UniProt and/or Google AI.