Product Name:
HGK-NT
Product Number:
ab-nk300-1
Target Full Name: Mitogen-activated protein kinase kinase kinase kinase 4
Target Alias: FLH21957; Hepatocyte progenitor kinase-like/germinal center kinase-like kinase; HGK; HPK/GCK-like kinase HGK; M4K4; MAP4K4; MAPK kinase kinase kinase 4; MAPK/ERK kinase kinase kinase 4; MEK kinase kinase 4; MEKKK 4; MEKKK4; Mitogen-activated protein kinase kinase kinase kinase 4; Nck interacting kinase; NIK; FLJ10410; FLJ20373; FLJ90111; KIAA0687; NIK; O95819; ENSG0000007105
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK300-1
Antibody Target Type: Pan-specific
Protein UniProt: O95819
Protein SigNET: O95819
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human HGK (ZC1) sequence peptide Cat. No.: PE-01BFM
Antibody Immunogen Sequence: ANDSPAKSLVDIDLSSLRDC
Antibody Immunogen Description: Corresponds to amino acid residues A2 to D20; N-terminus
Production Method: Corresponds to amino acid residues A2 to D20; N-terminus
Antibody Modification: Protein kinase pan-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 135-150 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: No significant cross-reactivities detected in various human cancer cell lines, including A431, HEK-293, HeLa, HepG2, Jurkat and MCF7 cells, except for very strong ~82 kDa and ~25-26 kDa cross-reactive proteins. No cross-reactivities detected in mouse tissues, except for very strong ~85 and ~90 kDa proteins in mouse brain, skeletal muscle and testes.
Related Product 1: HGK-NT blocking peptide
Related Product 2: HGK-pT187 phosphosite-specific antibody (Cat. No.: AB-PK653)
Scientific Background: ZC1 (HGK, MAP4K4) is a protein-serine/threonine kinase of the STE group and STE20 family. It may play a role in the response to environmental stress and cytokines such as TNF-alpha, and appears to act upstream of the JUN N-terminal pathway. ZC1 can phosphorylate SMAD1 at T322. Insertional mutagenesis studies in mice support a role for this protein kinase in mouse cancer oncogenesis.