Product Name:
ULK1
Product Number:
ab-nk298-1
Target Full Name: Unc 51 (C. elegans) like kinase 1
Target Alias: Unc-51 like kinase 1 (C. elegans); Autophagy related protein 1 homologue; Autophagy-related protein 1 homologue; FLJ38455; Serine/threonine-protein kinase ULK1; Unc51.1; ATG1A; UNC51; C. elegans; homologue of; EC:2.7.11.1; Serine/threonine protein kinase Unc51.1; FLJ46475; ATG 1; hATG1; ATG1; Unc-51-like kinase 1; Serine/threonine protein kinase ULK1; ULK1; Unc 51 like kinase 1; KIAA0722; UNC 51; ULK 1; ULK1_HUMAN; UNC51; ATG1 autophagy related 1 homologue;
Product Type Specific: ULK1 protein kinase pan-specific antibody
Antibody Code: NK298-1
Antibody Target Type: Pan-specific
Protein UniProt: O75385
Protein SigNET: ULK1
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide patterned after the mid-protein of human ULK1
Antibody Immunogen Sequence: RPKLPKPPTDP
Antibody Immunogen Description: Corresponds to amino acid residues R567 to P577.
Production Method: Peptide Affinity purified
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.25 mg/ml
Storage Buffer: Phosphate buffered saline, 50% glycerol, 0.09% sodium azide
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting
Antibody Dilution Recommended: WB (1:1000); optimal dilutions for assays should be determined by the user.
Antibody Potency: Detects a medium-weak ~95 kDa protein band in rat brain lysates, and a medium ~113 kDa protein band in HeLa cell lysates.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Dog | Rat | Mouse.
Antibody Positive Control: A 1:1000 dilution of SPC-640 was sufficient for detection of ULK1 in 15 µg of Human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody Specificity: Very high
Antibody Cross Reactivity: No other prominent cross-reactive proteins in mouse brain lysates.
Related Product 1: ULK2 pan-specific antibody (Cat. No.: AB-NK354-1)
Related Product 2: ULK3 pan-specific antibody (Cat. No.: AB-NK355-1)
Scientific Background: ULK1 (UNC-51 like kinase 1) is a ~112-kDa serine/threonine protein kinase that acts as the master initiator of autophagy in mammals, serving as the orthologue to yeast Atg1. It forms a core complex with ATG13, FIP200, and ATG101 to trigger autophagosome formation in response to nutrient starvation, regulated by mTORC1 (inhibition) and AMPK (activation). ULK1 phosphorylates key components like BECN1 and VPS34 to initiate autophagy. It is crucial for selective autophagy, including mitophagy (mitochondrial clearance). When nutrients are plentiful, mTORC1 phosphorylates and inactivates ULK1. During starvation/energy stress, AMPK phosphorylates and activates ULK1. It is widely expressed and contains an amino-terminal kinase domain followed by a central proline-serine rich domain and a highly conserved carboxy-terminal domain. It has been linked to axon growth and is essential for autophagy. Structurally, ULK1 is similar to ATG1, and it appears that both Atg1/ULK1 can bind to several ATG proteins regulating phosphorylation states and protein trafficking. ULK1 is heavily involved in autophagy regulation for cell survival, playing a key role in cancer progression, neurodegenerative diseases (clearance of protein aggregates), and heart disease. This description may include information annotated by UniProt and/or Google AI.