Product Name:
SRPK2-IKCD
Product Number:
ab-nk296-1
Target Full Name: Serine/arginine-rich protein-specific kinase 2; Serine kinase SRPK2
Target Alias: FLJ36101; Serine/arginine-rich protein-specific kinase 2; Serine/threonine-protein kinase SRPK2; SFRS protein kinase 2; SFRSK2; SR-protein-specific kinase 2; CCDS5735.1; ENSG00000135250
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK296-1
Antibody Target Type: Pan-specific
Protein UniProt: P78362
Protein SigNET: P78362
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human SRPK2 sequence peptide Cat. No.: PE-01BFI
Antibody Immunogen Sequence: CGEAEDQEEKEDAEKENIEK
Antibody Immunogen Description: Corresponds to amino acid residues G343 to K361; Insert into kinase domain
Production Method: Corresponds to amino acid residues G343 to K361; Insert into kinase domain
Antibody Modification: Protein kinase pan-specific antibody
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 105-115 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactivities detected in various human cancer cell lines, including A431, HEK-293, HeLa, HepG2, Jurkat and MCF7 cells, or in mouse brain, skeletal muscle, testes and thymus.
Related Product 1: SRPK2-IKCD blocking peptide
Related Product 2: SRPK1-pS222 phosphosite-specific antibody (Cat. No.: AB-PK819)
Related Product 3: SRPKSubtide - SRPK1 protein kinase substrate peptide
Scientific Background: SRPK2 (SFRSK2) is one of several protein kinases that can phosphorylate KinSubaRRGSF. It is a protein-serine/threonine kinase of the CMGC group and SRPK family. This kinase is moderate to highly expressed in most tested human tissues. It is a member of the serine/arginine (SR) protein-specific kinase family that are cell cycle-regulated protein kinases which phosphorylate SR domain-containing proteins in nuclear speckles and mediate the pre-mRNA splicing events. It phosphorylates SFRS1 and SFRS2 on serine residues. It is involved in spliceosome assembly and splicing factor trafficking. SRPK2 is activated by phosphorylation at S52 and S588. SRPK2 knock down results in hypophosphorylation of the serine/arginine domain-containing human PRP28 protein thereby destabilizing PRP28 association with the tri-snRNP. RNAi-mediated depletion in HeLa cells showed that SRPK2 is essential for cell viability, and it is required for spliceosomal B complex formation. SRPK2 has been linked with the development of glioblastoma multiforme (GM).
