Product Name:
PRKY-ANT
Product Number:
ab-nk293-1
Target Full Name: Serine/threonine-protein kinase PRKY
Target Alias: Protein kinase, Y-linked
Product Type Specific: PRKY protein kinase pan-specific antibody
Antibody Code: NK293-1
Antibody Target Type: Pan-specific
Protein UniProt: O43930
Protein SigNET: PRKY
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Synthetic peptide patterned after human PRKY
Antibody Immunogen Sequence: CAESNSREVTEDAADWAPAL
Antibody Immunogen Description: Corresponds to amino acid residues A12 to L30.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque.
Related Product 1: PRKX expression antibody (Cat. No.: AB-NK292-1)
Related Product 2: PRKX T201+T203 phosphorylation antibody (Cat. No.: AB-PK785)
Scientific Background: PRKY (Protein Kinase, Y-linked) is a primate-specific, Y-chromosome-encoded protein kinase, highly homologous to the X-linked PRKX. It belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family, cAMP subfamily. It is located near the pseudoautosomal region, and it acts as a serine/threonine protein kinase or a pseudo-kinase. Its promoter hypermethylation is a biomarker for early prostate cancer diagnosis, often showing reduced expression in affected tissues. Abnormal recombination between PRKY and PRKX (X-chromosome) during meiosis is associated with sex reversal, specifically accounting for roughly one-third of XX male and XY female cases. The promoter of the PRKY gene undergoes hypermethylation in prostate cancer, leading to reduced expression. This description may include information annotated by UniProt and/or Google AI.