Product Name:
Plk4-MID
Product Number:
ab-nk291-1
Target Full Name: Polo-like kinase-serine 4 (SAK)
Target Alias: Plk-4; Polo-like kinase 4; Sak; Serine,threonine-protein kinase 18; Serine/threonine protein kinase Sak; Serine/threonine-protein kinase 18; STK18; CCDS3735.1; O00444; ENSG00000142731
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK291-1
Antibody Target Type: Pan-specific
Protein UniProt: O00444
Protein SigNET: O00444
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Plk4 (SAK; STK18) sequence peptide Cat. No.: PE-01BFB
Antibody Immunogen Sequence: EKYWRKYQYASRFVQLVRSC
Antibody Immunogen Description: Corresponds to amino acid residues E680 to S698; Middle sequence
Production Method: Corresponds to amino acid residues E680 to S698; Middle sequence
Antibody Modification: Protein kinase pan-specific antibody
Antibody Concentration: 0.32 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Very weak detection of appropriately sized protein in A431 and HEK-293 cells.
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 100-110 kDa.
Antibody Specificity: High-very high
Antibody Cross Reactivity: No significant cross-reactivities detected in various human cancer cell lines, including A431, HEK-293, HeLa, HepG2, Jurkat and MCF7 cells, or in diverse mouse tissues.
Related Product 1: Plk4-MID blocking peptide
Related Product 2: Plk4-pT170 phosphosite-specific antibody (Cat. No.: AB-PK780)
Scientific Background: Plk4 (SAK) is a protein-serine/threonine kinase of the Other group and Plk family. It has a key function in centriole duplication through the recruitment of the centriole biogenesis proteins SASS6, CENPJ/CPAP, CCP110, CEP135, and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. It phosphorylates S151 of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6. It is involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activation of HAND1. It also phosphorylates CDC25C and Chk2 (CHEK2). Its central role in centriole replication indicates that it may have a possible role in tumourigenesis, as centrosome aberrations are frequently observed in tumours.