Product Name:
PKG2-AcNMP2
Product Number:
ab-nk290-1
Target Full Name: cGMP-dependent protein kinase 2
Target Alias: cGK 2; cGKII; KGP2; Kinase cGMP-dependent protein kinase 2PKG2; PRKG2; PRKGR2; Protein kinase, cGMP-dependent, type II; Type II cGMP-dependent protein kinase
Product Type Specific: PKG2 (PRKG2) protein kinase pan-specific antibody
Antibody Code: NK290-1
Antibody Target Type: Pan-specific
Protein UniProt: Q13237
Protein SigNET: PKG2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PKG2 (PRKG2) sequence peptide Cat. No.: PE-01BEW90
Antibody Immunogen Sequence: LQKYLEGYVANLNRDDEKRC
Antibody Immunogen Description: Corresponds to amino acid residues L397 to R415; Post-cNMP2.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.75 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 78-85 kDa.
Antibody Specificity: Medium-High
Antibody Cross Reactivity: Strong cross-reactivities for ~24, ~26 and~ 29 kDa proteins detected in mouse brain, testes and thymus. The ~24 and 26 kDa proteins were also strongly detected in human cancer cell lines, including A431, HEK-293, Jurkat and MCF7 cells.
Related Product 1: PKG2 (397-415) antibody blocking peptide (Cat. No.: PE-01BEW90)
Related Product 2: PKG2Subtide kinase substrate peptide (Cat. No.: PE-01BIX99)
Related Product 3: PKG1 expression antibody (Cat. No.: AB-NK202-2)
Related Product 4: PKG1 T515+T517 phosphorylation antibody (Cat. No.: AB-PK776)
Related Product 5: PKG1a expression antibody (Cat. No.: AB-NK202)
Scientific Background: PKG2 (PRKG2) is an ~87 kDa protein-serine/threonine kinase of the AGC group and PKG family. It acts as a membrane-bound, cGMP-dependent receptor to mediate critical cellular signalling, particularly in intestinal water/electrolyte transport, renin release from kidney juxtaglomerular cells and endochondral ossification (bone growth). Its deficiency is associated with dwarfism and skeletal dysplasia. PRKG2 phosphorylates target proteins (e.g., CFTR, Sox9) in response to increased intracellular cGMP levels. It also plays a role in synaptic plasticity by acting downstream of NMDAR. PRKG2 is highly expressed in cells like chondrocytes, intestinal mucosa, and certain brain neurons. Variants of the PRKG2 gene are linked to Acromesomelic Dysplasia 4, Spondylometaphyseal Dysplasia (Pagnamenta Type), and general skeletal defects. PKG2 may be a target in the treatment of colon cancer. This description may include information annotated by UniProt and/or Google AI.