Product Name:
Pim3-ANT
Product Number:
ab-nk289-1
Target Full Name: Serine/threonine-protein kinase Pim-3
Target Alias: Pim-3 oncogene; ENSG00000198355
Product Type Specific: Pim3 protein kinase pan-specific antibody
Antibody Code: NK289-1
Antibody Target Type: Pan-specific
Protein UniProt: Q86V86
Protein SigNET: Pim3
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human Pim3 sequence peptide Cat. No.: PE-01BET85
Antibody Immunogen Sequence: CVKILQPAKADKESFEKAYQ
Antibody Immunogen Description: Corresponds to amino acid residues V23 to Q41; Post-N-terminus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity of a target-sized protein by Western blotting in various mouse tissues.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Dog | Rat | Mouse | Frog.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 30-34 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactivities detected in various human cancer cell lines, including A431, HEK-293, HeLa, HepG2, Jurkat and MCF7 cells, except for a medium ~49 kDa protein in A431 cells. No cross-reactivities detected in mouse brain, skeletal muscle, testes and thymus.
Related Product 1: PIM3 (23-41) antibody blocking peptide (Cat. No.: PE-01BET85)
Related Product 2: PimSelectide - Pim1 protein kinase substrate peptide
Related Product 3: RPS6 (230-238) KinSub kinase substrate peptide (Cat. No.: PE-01ADQ95)
Related Product 4: Pim1 expression antibody (Cat. No.: AB-NK258-2)
Related Product 5: Pim2 expression antibody (Cat. No.: AB-NK288-1)
Related Product 6: Pim2 expression antibody (Cat. No.: AB-NK288-2)
Related Product 7: Pim2 T195 phosphorylation antibody (Cat. No.: AB-PK761)
Scientific Background: Pim3 is a protein-serine/threonine kinase of the CAMK group and Pim family. The Pim3 protein, along with the related Pim1 and Pim2 proteins, facilitates cell survival and proliferation by acting downstream of cytokine and growth factor signalling pathways. It can prevent apoptosis, and promote cell survival and protein translation. It appears to be a tumour requiring protein (TRP), since it undergoes fewer mutations than the typical protein in cancer cells. The active form of the protein kinase normally acts to promote tumour cell proliferation. Pim3 promotes tumorigenesis, and is upregulated in human pancreatic cancer tissue and pancreatic cancer cell lines. This description may include information annotated by UniProt and/or Google AI.