Product Name:
PCTK3-ANT
Product Number:
ab-nk286-1
Target Full Name: Cell division protein kinase 18; Protein-serine kinase PCTAIRE-3
Target Alias: CDK18; KPT3; PCTAIRE protein kinase 3; PCTK3; Serine/threonine-protein kinase PCTAIRE-3; ENSG00000117266
Product Type Specific: PCTAIRE3 (CDK18) protein kinase pan-specific antibody
Antibody Code: NK286-1
Antibody Target Type: Pan-specific
Protein UniProt: Q07002
Protein SigNET: PCTK3
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human PCTK3 (PCTAIRE3, CDK18) sequence peptide Cat. No.: PE-01BEK65
Antibody Immunogen Sequence: CIEESLAEFTEQFNQLHNRR
Antibody Immunogen Description: Corresponds to amino acid residues I21 to R39; Post-N-terminus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Potency: Strong immunoreactivity of a target-sized protein by Western blotting in various human cancer cell lines, including A431, HEK-293, HeLa, HepG2, Jurkat and MCF7 cells, and in mouse brain, testes and thymus.
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Dog | Rat | Mouse | Platypus | Chicken.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 50-55 kDa.
Antibody Specificity: Medium
Antibody Cross Reactivity: Strong cross-reactivities for ~110 and ~74 kDa detected in various human cancer cell lines, including A431, HEK-293, HeLa, HepG2, Jurkat and MCF7 cells. Medium-strong cross-reactivities with 5 proteins in mouse brain and testes and thymus.
Related Product 1: PCTAIRE3 (21-39) antibody blocking peptide (Cat. No.: PE-01BEK65)
Related Product 2: PCTK1 Y176 phosphorylation antibody (Cat. No.: AB-PK755)
Related Product 3: PCTK2 expression antibody (Cat. No.: AB-NK285-1)
Related Product 4: PCTK2 S180 phosphorylation antibody (Cat. No.: AB-PK756)
Scientific Background: PCTK3 (PCTAIRE3, CDK18) is a protein-serine/threonine kinase of the CMGC group and CDK family. It may play a role in terminally differentiated cells. It is predominantly expressed in the brain and other post-mitotic cells, where it plays a key role in regulating cytoskeleton dynamics, specifically by controlling actin reorganization. PCTK3 is involved in cell migration, adhesion, and regulation of the actin cytoskeleton by negatively regulating the FAK/RhoA signalling pathway. It phosphorylate retinoblastoma protein (Rb) and regulate the stability of cyclin A2. It is primarily found in the cytoplasm and can colocalize with cyclin A2. PCTK3 is activated through two primary pathways: binding with cyclin A2 and phosphorylation by protein kinase A (PKA) at Ser-12. It features a central kinase domain (highly conserved) and unique N- and C-terminal domains that differ from other CDKs. Due to its expression in the brain, it is linked to studies on neuronal function and potential involvement in neurodegenerative diseases like Alzheimer's. This description may include information annotated by UniProt and/or Google AI.