Product Name:
MRCKa-AKCD
Product Number:
ab-nk282-1
Target Full Name: Myotonic dystrophy kinase-related CDC42-binding kinasealpha
Target Alias: CDC42BPA; CDC42-binding protein kinase alpha; DMPK-like alpha; DKFZp686L1738; FLJ23347; KIAA0451; MRCK alpha; PK428
Product Type Specific: MRCKa (CDC42BPA) protein kinase pan-specific antibody
Antibody Code: NK282-1
Antibody Target Type: Pan-specific
Protein UniProt: Q5VT25
Protein SigNET: MRCKa
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MRCKa (PK428) sequence peptide Cat. No.: PE-01BED70
Antibody Immunogen Sequence: CVLSDRSSLRVTAGPTSLDL
Antibody Immunogen Description: Corresponds to amino acid residues C408 to L427; Right after kinase catalytic domain.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Dog | Rat | Mouse | Playtpus.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 190-210 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactivities detected in various human cancer cell lines, including A431, HEK-293, HepG2, Jurkat and MCF7 cells, except for a weak ~45 kDa protein in No cross-reactivities detected in mouse brain, skeletal muscle, testes and thymus, except for a medium ~100 kDa protein in mouse brain.
Related Product 1: MRCKa (408-427) antibody blocking peptide (Cat. No.: PE-01BED70)
Scientific Background: MRCK-a (CDC42BPA; Myotonic dystrophy kinase-related Cdc42-binding kinase alpha) is a 180 kDa protein-serine/threonine kinase of the AGC group and DMPK family. It is a critical downstream effector of the Cdc42 GTPase and plays a role in the regulation of cytoskeleton remodelling and cell migration. MRCK-a contains an N-terminal kinase domain, a central coiled-coil region for dimerization, and a C-terminal CRIB (Cdc42/Rac interaction binding) domain. Agonist binding to the phorbol ester binding site disrupts this, releasing the kinase domain to allow N-terminus-mediated dimerization and kinase activation by transautophosphorylation. MRCK-a is maintained in an inactive, closed conformation by an interaction between the kinase domain and the negative autoregulatory C-terminal coiled-coil region. It is regulates myosin-based contractility, especially in cancer cell invasion and epithelial development. MRCK-a regulates actin remodeling by phosphorylating substrates like Myosin Light Chain (MLC2) and its regulator, MYPT1. In cell migration, it functions in the formation of lamellipodia and cell protrusion, working in concert with factors like LURAP1. It is involved in apical domain organization, tissue morphogenesis, and epithelial extrusion. It is ubiquitously expressed, with high expression levels found in the heart, brain, skeletal muscle, kidney, and pancreas. It is often located with the plasma membrane, particularly at the leading edge of migrating cells (lamellipodium) and at the Golgi apparatus. This description may include information annotated by UniProt and/or Google AI.