Product Name:
MRCKa-AKCD
Product Number:
ab-nk282-1
Target Full Name: Myotonic dystrophy kinase-related CDC42-binding kinasealpha
Target Alias: CDC42BPA; CDC42-binding protein kinase alpha; DMPK-like alpha; DKFZp686L1738; FLJ23347; KIAA0451; MRCK alpha; PK428
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK282-1
Antibody Target Type: Pan-specific
Protein UniProt: Q5VT25
Protein SigNET: Q5VT25
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MRCKa (PK428) sequence peptide Cat. No.: PE-01BED70
Antibody Immunogen Sequence: CVLSDRSSLRVTAGPTSLDL
Antibody Immunogen Description: Corresponds to amino acid residues C408 to L427; Right after kinase catalytic domain
Production Method: Corresponds to amino acid residues C408 to L427; Right after kinase catalytic domain
Antibody Modification: Protein kinase pan-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 190-210 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactivities detected in various human cancer cell lines, including A431, HEK-293, HepG2, Jurkat and MCF7 cells, except for a weak ~45 kDa protein in No cross-reactivities detected in mouse brain, skeletal muscle, testes and thymus, except for a medium ~100 kDa protein in mouse brain.
Related Product 1: MRCKa-AKCD blocking peptide
Scientific Background: MRCK-a (CDC42BPA, CDC42-binding protein kinase A) is a protein-serine/threonine kinase of the AGC group and DMPK family. It is a critical downstream effector and plays a role in the regulation of cytoskeleton remodelling and cell migration. Agonist binding to the phorbol ester binding site disrupts this, releasing the kinase domain to allow N-terminus-mediated dimerization and kinase activation by transautophosphorylation. MRCK-a is maintained in an inactive, closed conformation by an interaction between the kinase domain and the negative autoregulatory C-terminal coiled-coil region.