Product Name:
MLK4-CT
Product Number:
ab-nk280-1
Target Full Name: Mixed-lineage protein-serine kinase 4
Target Alias: M3KL4; Mixed lineage kinase 4; KIAA1804
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK280-1
Antibody Target Type: Pan-specific
Protein UniProt: Q5TCX8
Protein SigNET: Q5TCX8
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MLK4 sequence peptide Cat. No.: PE-01BEA90
Antibody Immunogen Sequence: CMRSKTSRPSIYELEKEFLS
Antibody Immunogen Description: Corresponds to amino acid residues M1018 to S1036; C-terminus
Production Method: Corresponds to amino acid residues M1018 to S1036; C-terminus
Antibody Modification: Protein kinase pan-specific antibody
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 95-120 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No significant cross-reactivities detected in various human cancer cell lines, including A431, HEK-293, HeLa, HepG2, Jurkat and MCF7 cells. No significant cross-reactive proteins detected in diverse mouse tissues, except a medium ~30 kDa protein in mouse brain.
Related Product 1: MLK4-CT blocking peptide
Scientific Background: MLK4 (M3KL4) is a protein-serine/threonine kinase of the TKL group and MLK family. It acts to impair TLR4 signalling, and will not activate any of the JNK1/MAPK8, or p38/MAPK14, or ERK2/MAPK1 pathways. Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation. Mutations in MLK4 have been linked to Colorectal cancer (CRC).