Product Name:
MARK4-ANT
Product Number:
ab-nk277-1
Target Full Name: MAP/microtubule affinity-regulating protein-serine kinase 4
Target Alias: FLJ90097; KIAA1860; Kinase MARK4; MARKL1; MARKL1, Nbla00650; CCDS12658.1; ENSG00000007047
Product Type Specific: MARK4 protein kinase pan-specific antibody
Antibody Code: NK277-1
Antibody Target Type: Pan-specific
Protein UniProt: Q96L34
Protein SigNET: MARK4
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MARK4 sequence peptide Cat. No.: PE-01BDV80
Antibody Immunogen Sequence: CGSGRSSDKGPSWSSRSLGA
Antibody Immunogen Description: Corresponds to amino acid residues G22 to C40; Post-N-terminus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 75-85 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No cross-reactivities detected in mouse brain, skeletal muscle, testes and thymus.
Related Product 1: MARK4 (22-40) antibody blocking peptide (Cat. No.: PE-01BDV80)
Related Product 2: MARK1-ANT pan-specific antibody (Cat. No.: AB-NK098-2)
Related Product 3: MARK1-pT215 phosphosite-specific antibody (Cat. No.: AB-PK694)
Related Product 4: MARK1-pT215 phosphosite-specific antibody (Cat. No.: AB-PK695)
Related Product 5: MARK2-ANT pan-specific antibody (Cat. No.: AB-NK275-1)
Related Product 6: MARK2-BCT pan-specific antibody (Cat. No.: AB-NK275-2)
Related Product 7: MARK3-ANT pan-specific antibody (Cat. No.: AB-NK276-1)
Related Product 8: MARK3-BCT pan-specific antibody (Cat. No.: AB-NK276-2)
Related Product 9: MARK3-pT507 phosphosite-specific antibody (Cat. No.: AB-PK697)
Related Product 10: MARK4-NT pan-specific antibody (Cat. No.: AB-NK277-2)
Scientific Background: MARK4 (Microtubule Affinity-Regulating Kinase 4) is a protein-serine/threonine kinase of the CAMK group and CAMKL family, which regulates microtubule dynamics by phosphorylating microtubule-associated proteins (MAPs), causing them to detach from microtubules. It features an N-terminal catalytic domain, an autoregulatory UBA domain, a spacer region, and a C-terminal tail. It exists in two isoforms: long (MARK4L) and short (MARK4S), with differing expression patterns. It is primarily expressed in the brain and testis. MARK4 is generally found in the cytoplasm, localized at the centrosome and midbody, particularly during cell division. It is associated with the centrosome during mitosis. It is crucial for maintaining microtubule-organizing center (MTOC) function. It phosphorylates tau protein and other MAPs. MARK4 levels are elevated in Alzheimer's disease, contributes to pathological phosphorylation of tau protein. Reduction in MARK4 levels has been shown to protect against diet-induced obesity in mice. Mutation in this gene have been found in gliomas, mouth and ovarian cancers. This description may include information annotated by UniProt and/or Google AI.