Product Name:
MARK3-ANT
Product Number:
ab-nk276-1
Target Full Name: MAP/microtubule affinity-regulating protein-serine kinase 3
Target Alias: Cdc25C- associated protein kinase 1; Cdc25C-associated protein kinase 1; CTAK1; C-TAK1; ELKL motif kinase 2; KP78; PAR1A; Protein kinase STK10; Ser/Thr protein kinase PAR-1; Serine/threonine protein kinase p78; Serine/threonine-protein kinase p78
Product Type Specific: MARK3 protein kinase pan-specific antibody
Antibody Code: NK276-1
Antibody Target Type: Pan-specific
Protein UniProt: P27448
Protein SigNET: MARK3
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MARK3 sequence peptide Cat. No.: PE-01BDT90
Antibody Immunogen Sequence: HGDGRQEVTSRTSRSGARC
Antibody Immunogen Description: Corresponds to amino acid residues H21 to C39; Post-N-terminus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Dog | Rat | Mouse | Platytpus | Frog.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 80-85 kDa.
Related Product 1: MARK3 (21-39) antibody blocking peptide (Cat. No.: PE-01BDT90)
Related Product 2: MARK1-ANT pan-specific antibody (Cat. No.: AB-NK098-2)
Related Product 3: MARK1-pT215 phosphosite-specific antibody (Cat. No.: AB-PK694)
Related Product 4: MARK1-pT215 phosphosite-specific antibody (Cat. No.: AB-PK695)
Related Product 5: MARK2-ANT pan-specific antibody (Cat. No.: AB-NK275-1)
Related Product 6: MARK2-BCT pan-specific antibody (Cat. No.: AB-NK275-2)
Related Product 7: MARK3-BCT pan-specific antibody (Cat. No.: AB-NK276-2)
Related Product 8: MARK3-pT507 phosphosite-specific antibody (Cat. No.: AB-PK697)
Related Product 9: MARK4-ANT pan-specific antibody (Cat. No.: AB-NK277-1)
Related Product 10: MARK4-NT pan-specific antibody (Cat. No.: AB-NK277-2)
Scientific Background: MARK3 (Microtubule Affinity Regulating Kinase 3; C-TAK1) is a ~110 kDa protein-serine/threonine kinase of the CAMK group and CAMKL family, and regulates cell polarity, microtubule stability, and cell cycle progression. It phosphorylates microtubule-associated proteins (MAP2, MAP4, TAU), reducing their affinity for microtubules, and acts as a key mediator in the Hippo signaling pathway by inhibiting LATS1. It features an N-terminal kinase domain, a UBA (ubiquitin-associated) domain, and a C-terminal spacer. Its activity is controlled by phosphorylation from upstream kinases like LKB1 (serine/threonine-protein kinase STK11) and interaction with 14-3-3 scaffolding proteins. Phosphorylation of Thr-234 and Ser-238 in MARK3 increases phosphotransferase activity. T211A substitution is involved in decreased phosphorylation and activation by LKB1/LKB1 complex. Phosphorylation of Thr-95 and Ser-96 inhibits phosphotransferase activity. MARK3 phosphorylates CDC25C on Ser-216, controlling the G2/M transition of the cell cycle. It also plays a role in regulating the localization of histone deacetylases (HDAC7) and MITF. MARK3 regulates bone mineral density. It is also associated with carcinogenesis, particularly in the pancreas, and has been identified in a form of genetic recessive blindness. This description may include information annotated by UniProt and/or Google AI.