Product Name:
MARK2-BCT
Product Number:
ab-nk275-2
Target Full Name: MAP/microtubule affinity-regulating protein-serine kinase 2
Target Alias: ELKL motif kinase 1; EMK; EMK1; Kinase MARK2; MAP/microtubule affinity-regulating kinase 2; MGC99619; PAR-1; Par1b; PAR-1b; Serine/threonine kinase
Product Type Specific: MARK2 protein kinase pan-specific antibody
Antibody Code: NK275-2
Antibody Target Type: Pan-specific
Protein UniProt: Q7KZI7
Protein SigNET: MARK2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MARK2 sequence peptide Cat. No.: PE-01BDS80
Antibody Immunogen Sequence: CGGNDKEKEEFREAKP
Antibody Immunogen Description: Corresponds to amino acid residues G676 to P690; Pre-C-terminus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Dog | Rat | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 80-90 kDa.
Related Product 1: MARK2 (676-690) antibody blocking peptide (Cat. No.: PE-01BDS80)
Related Product 2: MARK1-ANT pan-specific antibody (Cat. No.: AB-NK098-2)
Related Product 3: MARK1-pT215 phosphosite-specific antibody (Cat. No.: AB-PK694)
Related Product 4: Chk1Selectide kinase substrate peptide (Cat. No.: PE-01BGS90)
Related Product 5: MARK1-pT215 phosphosite-specific antibody (Cat. No.: AB-PK695)
Related Product 6: MARK2-ANT pan-specific antibody (Cat. No.: AB-NK275-1)
Related Product 7: MARK3-ANT pan-specific antibody (Cat. No.: AB-NK276-1)
Related Product 8: MARK3-BCT pan-specific antibody (Cat. No.: AB-NK276-2)
Related Product 9: MARK3-pT507 phosphosite-specific antibody (Cat. No.: AB-PK697)
Related Product 10: MARK4-ANT pan-specific antibody (Cat. No.: AB-NK277-1)
Scientific Background: MARK2 (Microtubule Affinity Regulating Kinase 2; Par-1b) is a protein-serine/threonine kinase of the CAMK group and CAMKL family. MARK2 is crucial for establishing and maintaining apical-basal polarity in epithelial cells and polarization in neurons. It features a catalytic kinase domain, a ubiquitin-associated (UBA) domain, and a kinase-associated domain 1 (KA1) that binds phospholipids. It is activated by phosphorylation on Thr-208 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. It mediates cell polarity and microtubule function. Some substrate proteins that MARK2 phosphorylates include subunits of the mTOR/HIF-1 pathway CRTC2/TORC2, HDAC7, DCX, KIF13B, MAPT/TAU, MAP2, MAP4, and RAB11FIP2. It acts as a positive regulator of the Wnt signaling pathway. Phosphorylation of microtubule associated proteins leads to protein dissociation and microtubule degeneration. It promotes microtubule instability, which is critical for remodeling the cytoskeleton. It also acts in the actin network to stabilize the mitotic spindle and regulate cell division. The phosphorylation of MARK2 by the LKB1/LKB1 complex can occur with a T208A mutation in MARK2. The ability of MARK2 to bind YWHAZ and to dissociate from the membrane occurs with a T596A mutation. Inhibition of MARK2 via small inhibitory RNA induced axon-like outgrowth from neurites. MARK2 has been implicated in cancer progression (e.g., breast cancer, non-small cell lung carcinoma) through the modulation of metabolism and growth signalling, such as the Warburg effect. It also plays a role in neuronal development and may be involved involved in Alzheimer's neurofibrillary pathology by phosphorylating Tau. This description may include information annotated by UniProt and/or Google AI.