Product Name:
MARK2-ANT
Product Number:
ab-nk275-1
Target Full Name: MAP/microtubule affinity-regulating protein-serine kinase 2
Target Alias: ELKL motif kinase 1; EMK; EMK1; Kinase MARK2; MAP/microtubule affinity-regulating kinase 2; MGC99619; PAR-1; Par1b; PAR-1b; Serine/threonine kinase
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK275-1
Antibody Target Type: Pan-specific
Protein UniProt: Q7KZI7
Protein SigNET: Q7KZI7
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human MARK2 sequence peptide Cat. No.: PE-01BDR80
Antibody Immunogen Sequence: PTLGHLDSKPSSKSNMIRGC
Antibody Immunogen Description: Corresponds to amino acid residues P19 to G37; Post-N-terminus
Production Method: Corresponds to amino acid residues P19 to G37; Post-N-terminus
Antibody Modification: Protein kinase pan-specific antibody
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 80-90 kDa.
Related Product 1: MARK2-ANT blocking peptide
Related Product 2: MARK1-ANT pan-specific antibody (Cat. No.: AB-NK098-2)
Related Product 3: MARK1-pT215 phosphosite-specific antibody (Cat. No.: AB-PK694)
Related Product 4: Cdc25C (205-225), KinSub - Cdc25C phosphatase (V205-R225, human) peptide; Chktide peptide
Related Product 5: MARK1-pT215 phosphosite-specific antibody (Cat. No.: AB-PK695)
Related Product 6: MARK2-BCT pan-specific antibody (Cat. No.: AB-NK275-2)
Related Product 7: MARK3-ANT pan-specific antibody (Cat. No.: AB-NK276-1)
Related Product 8: MARK3-BCT pan-specific antibody (Cat. No.: AB-NK276-2)
Related Product 9: MARK3-pT507 phosphosite-specific antibody (Cat. No.: AB-PK697)
Related Product 10: MARK4-ANT pan-specific antibody (Cat. No.: AB-NK277-1)
Scientific Background: MARK2 is a protein-serine/threonine kinase of the CAMK group and CAMKL family. It is activated by phosphorylation on T208 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. It mediates cell polarity and microtubule function. Some substrate proteins that MARK2 phosphorylates include CRTC2/TORC2, HDAC7, DCX, KIF13B, MAPT/TAU, MAP2, MAP4, and RAB11FIP2. Phosphorylation of microtubule associated proteins leads to protein dissociation and microtubule degeneration. The phosphorylation of MARK2 by the LKB1/LKB1 complex can occur with a T208A mutation in MARK2. The ability of MARK2 to bind YWHAZ and to dissociate from the membrane occurs with a T596A mutation. Inhibition of MARK2 via small inhibitory RNA induced axon-like outgrowth from neurites.