Product Name:
IRR-BCT
Product Number:
ab-nk273-1
Target Full Name: Insulin receptor-related protein
Target Alias: INSRR; Insulin receptor-related protein precursor; IR-related receptor
Product Type Specific: IRR (INSRR) protein kinase pan-specific antibody
Antibody Code: NK273-1
Antibody Target Type: Pan-specific
Protein UniProt: P14616
Protein SigNET: IRR
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human IRR (INSRR) sequence peptide Cat. No.: PE-01BDJ85
Antibody Immunogen Sequence: RGSLPTTDAEPDSSPTPRDC
Antibody Immunogen Description: Corresponds to amino acid residues R1269 to C1288; Pre-C-terminus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.4 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 120-130 kDa.
Related Product 1: IRR (1269-1288) antibody blocking peptide (Cat. No.: PE-01BDJ85)
Related Product 2: IRS1 (979-989; mouse) KinSub kinase substrate peptide (Cat. No.: PE-01ADC95)
Related Product 3: IRS1 (891-902) KinSub - Insulin receptor substrate 1 (K891-G902) mouse, peptide; IGF1Rtide protein kinase substrate peptide
Scientific Background: IRR (INSRR) is a receptor protein-tyrosine kinase that is a member of the TK group of protein kinases in the InsR family. IRR exists on the cell surface as a preformed, disulfide-linked dimer (an heterotetramer). It has high homology (50-54% identity) with the insulin and IGF-1 receptors. Unlike its relatives that are activated by hormones, IRR is considered an "orphan receptor" because it does not bind to any known peptide or protein ligands, such as insulin or IGFs. IRR functions specifically as an extracellular pH sensor that is activated by alkaline conditions (pH > 7.9–8.0). It is mainly expressed in specialized tissues that come into contact with alkaline fluids, such as the kidney (specifically beta-intercalated cells), stomach, and pancreas. IRR is required for the appearance of male gonads and for male sexual differentiation. I When IRR is activated a signalling cascade is started which involves IRS1 and PKB/Akt1. A mutation that can inhibit IRR autophosphorylation is Y1145F with Y1146F. IRR-deficient (knockout) mice show defects in renal response to alkali loading (inability to properly excrete bicarbonate). Expression of IRR mRNA is restricted to a few tissue types and has been detected in neuronal tissues as well as in neuroblastomas. As a result of its occurrence in neuroblastomas, it has speculated that IRR may be involved in tumorigenesis. RT-PCR and solution hybridization/RNAse protection assay have shown strong coupling of IRR mRNA in 18 tumour cell lines. IRR has also been linked with the development of glioblastoma multiforme and lung adenocarcinomas. This description may include information annotated by UniProt and/or Google AI.