Product Name:
HIPK2-BCT
Product Number:
ab-nk272-1
Target Full Name: Homeodomain-interacting protein-serine kinase 2
Target Alias: Homeodomain interacting protein kinase 2; Kinase HIPK2; NBAK1; Nuclear body associated kinase 1; Sialophorin tail associated nuclear serine,threonine kinase; Sialophorin tail associated nuclear serine/threonine kinase; STANK; PRO0593; Q9H2X6; ENSG00000064393
Product Type Specific: HIPK2 protein kinase pan-specific antibody
Antibody Code: NK272-1
Antibody Target Type: Pan-specific
Protein UniProt: Q9H2X6
Protein SigNET: HIPK2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human HIPK2 sequence peptide Cat. No.: PE-01BDD99
Antibody Immunogen Sequence: HPSQYPAQFAHQTYISASC
Antibody Immunogen Description: Corresponds to amino acid residues H1159 to S1176; Pre-C-terminus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Dog | Rat | Mouse | Chicken | Zebra fish.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 120-125 kDa.
Related Product 1: HIPK2 (1159-1176) antibody blocking peptide (Cat. No.: PE-01BDD99)
Related Product 2: HIPK1 Y352 phosphorylation antibody (Cat. No.: AB-PK654)
Related Product 3: DYRKtide KinSub kinase substrate peptide (Cat. No.: PE-01ACQ95)
Scientific Background: HIPK2 is a nuclear protein-serine/threonine kinase of the CMGC group and DYRK family. It is activated by stress signals such as UV light, ionizing radiation, and genotoxic chemotherapy. It acts as a vital stress sensor, integrating DNA-damage, hypoxia, and TGF-beta signals to regulate cell fate, including apoptosis and transcription. It is involved in inducing apoptosis, through phosphorylation of p53, after genotoxic stress. It interacts with numerous transcription factors (such as p53, AML1 (RUNX1), PAX6, c-MYB or NK3) as well as transcriptional regulators (such as CBP, p300, Groucho, CtBP, HMGA1 or Smads). In this way HIPK2 can activate or repress transcription and influence differentiation, development and the DNA damage response. HIPK2 can promote the apoptotic program via p53-dependent and -independent pathways through phosphorylation of p53 at Ser-46 or phosphorylation of the anti-apoptotic co-repressor CtBP at Ser-422 (both actions leading to the transcription of pro-apoptotic target genes). HIPK2 contains an N-terminal kinase domain, a central homeobox-interacting domain, a speckle-retention signal (for localization to nuclear bodies), and a C-terminal auto-inhibitory domain. While HIPK2 is mostly a nuclear protein, concentrated in nuclear "speckles," but it can also be found in the cytoplasm. It is degraded by ubiquitin ligases (e.g., Siah1/2) in a regulated manner. Its activity is crucial for regulating cell death in neurodegenerative diseases (e.g., Alzheimer's disease) and driving fibrosis in kidney diseases. HIPK2 may be an oncoprotein (OP) or a tumour suppressor protein (TSP) depending on the circumstance. HIPK2 is upregulated in some cervical cancers, and down regulated in some thyroid and breast carcinomas. In cervical cancer, upregulated HIPK2 may represent a compensation of normal cellular machinery. In most cancer systems, it appears to repress growth and prevent tumorigenesis. This description may include information annotated by UniProt and/or Google AI.