Product Name:
GSK3b-ANT
Product Number:
ab-nk270-3
Target Full Name: Glycogen synthase-serine kinase 3 beta
Target Alias: Factor A; Glycogen synthase kinase 3 beta; GSK-3 beta; GSK3-beta; Kinase GSK-3 beta; Kinase GSK3-beta; Protein kinase GSK-3-beta; CCDS2996.1; ENSG00000082701
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK270-3
Antibody Target Type: Pan-specific
Protein UniProt: P49841
Protein SigNET: P49841
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human GSK3b sequence peptide Cat. No.: PE-01BDB85
Antibody Immunogen Sequence: CKPVQQPSAFGSMKVSRDKD
Antibody Immunogen Description: Corresponds to amino acid residues C14 to D33; Near N-terminus
Production Method: Corresponds to amino acid residues C14 to D33; Near N-terminus
Antibody Modification: Protein kinase pan-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 45-50 kDa.
Related Product 1: GSK3b-ANT blocking peptide
Related Product 2: GSK-3Beta pan-specific antibody (Cat. No.: AB-NK070-4)
Related Product 3: GSK3b-XI pan-specific antibody (Cat. No.: AB-NK194-NK195-3)
Related Product 4: GSK3b-XI (GSK3b-1) pan-specific antibody (Cat. No.: AB-NK070)
Related Product 5: GSK3a-pS278+pY279 phosphosite-specific antibody (Cat. No.: AB-PK647)
Related Product 6: GSK3a-pT19+pS21 phosphosite-specific antibody (Cat. No.: AB-PK648)
Related Product 7: GSK3a-pY279 phosphosite-specific antibody (Cat. No.: AB-PK649)
Related Product 8: GSK3a-pY284+Y285 phosphosite-specific antibody (Cat. No.: AB-PK650)
Related Product 9: GSK3Selectide - GSK3 protein kinase substrate peptide
Scientific Background: GSK3b is a protein-serine/threonine kinase of the CMGC group and GSK family. It is a critical enzyme in glycogen metabolism, and plays a role in pathological conditions such as cancer, diabetes, and neurological diseases. This kinase is highly expressed and widely distributed in most tested human tissues. Orthologues of GSK3b are amongst the most highly conserved protein kinases in animals, plants, fungi and unicellular eukaryotes. GSK3b is activated by phosphorylation at Y216 by MEK1 and MEK2, and inhibited by phosphorylation of S9. GSK3b phosphorylates and inhibits glycogen synthase. It is implicated in the regulation of several physiological processes, including control of glycogen and protein synthesis by insulin, and modulation of the transcription factors AP-1 and CREB. Transient transfection of human GSK3b into Chinese hamster ovary cells stably transfected with individual human tau isoforms leads to hyperphosphorylation of tau. GSK3b has been linked with the development of Alzheimer’s disease, bipolar disorder, and Type 2 diabetes. Mutations leading to upregulation of GSK3b are also implicated in ovarian, prostate, pancreatic, colorectal, neuroblastoma, and glioblastoma neoplasms, by positively regulating cyclin D1 and thus promoting cell cycle progression.
