Product Name:
EphB2-PCT
Product Number:
ab-nk267-2
Target Full Name: Ephrin type-B receptor 2 protein-tyrosine kinase
Target Alias: CEK5; DRT; Eph receptor B2; Eph3; Eph-3; EphB2; Ephrin type-B receptor 2; EphT3; Hek5; Tyro5; MGC87492; ENSG00000133216
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK267-2
Antibody Target Type: Pan-specific
Protein UniProt: P29323
Protein SigNET: P29323
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human EphB2 sequence peptide Cat. No.: PE-01BCV85
Antibody Immunogen Sequence: REIQGIFFKEDSHKESNDC
Antibody Immunogen Description: Corresponds to amino acid residues R1033 to C1051; Near C-terminus
Production Method: Corresponds to amino acid residues R1033 to C1051; Near C-terminus
Antibody Modification: Protein kinase pan-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 100-115 kDa.
Related Product 1: EphB2-PCT blocking peptide
Related Product 2: EphB1-pY594 phosphosite-specific antibody (Cat. No.: AB-PK609)
Related Product 3: EphB2-BKCD pan-specific antibody (Cat. No.: AB-NK267-1)
Related Product 4: EphB2-pY780 phosphosite-specific antibody (Cat. No.: AB-PK610)
Related Product 5: EphB3-pY600 phosphosite-specific antibody (Cat. No.: AB-PK611)
Related Product 6: EphSubtide - EphB1 protein kinase substrate peptide
Scientific Background: EphB2 is a protein-tyrosine kinase of the TK group and Eph family. It is a receptor kinase, and is activated by binding ephrin-B1, B2, or B3. Eph and Eph receptors are associated with nervous system development events, and lead to contact-dependent signalling through adjacent cells. Eph receptors are widely expressed in early development and rarely detected in adult tissues. EphB2 signalling has an important role in the development and structural organization of neurons. EphB2 is also involved in angiogenesis, palate development and inner ear development through endolymph production. EphB2 appears to be a tumour suppressor protein (TSP). The active form of the protein kinase normally acts to inhibit tumour cell proliferation. EphB2 mutations have been found in prostate cancer. Most prostate cancers are adenocarcinomas that derived from acini of the prostatic ducts. K1019X mutation was significantly more common in African American probands. Over-expression of EphB2 has been frequently detected in both well-differentiated adenocarcinomas (10 of 13) and poorly differentiated adenocarcinomas (9 of 14). EphB2 expression restricts the invasiveness of colorectal cancer cells into ephrin-B1 expressing cell populations, thus segregating cancer cells from normal cells.