Product Name:
DMPK2-NT
Product Number:
ab-nk265-1
Target Full Name: Myotonic dystrophy protein kinase 2; Serine-threonine-protein kinase MRCK gamma
Target Alias: MRCKG; Myotonic dystrophy protein kinase like protein ; CDC42BPG; HSMDPKIN
Product Type Specific: DMPK2 protein kinase pan-specific antibody
Antibody Code: NK265-1
Antibody Target Type: Pan-specific
Protein UniProt: Q6DT37
Protein SigNET: DMPK2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human DMPK2 (MRCK-g) sequence peptide Cat. No.: PE-01BCS80
Antibody Immunogen Sequence: MERRLRALEQLARGEAGGC
Antibody Immunogen Description: Corresponds to amino acid residues M1 to C19; N-terminus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Rhesus macaque | Dog | Mouse.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around n/a kDa.
Related Product 1: DMPK2 (1-19) antibody blocking peptide (Cat. No.: PE-01BCS80)
Related Product 2: DMPKSubtide kinase substrate peptide (Cat. No.: PE-01BHC90)
Related Product 3: DMPK1 expression antibody (Cat. No.: AB-NK264-1)
Scientific Background: DMPK2 (Dystrophia Myotonica Protein Kinase-2) is a protein-serine/threonine kinase of the AGC group and DMPK family. It is a shorter, soluble form of the DMPK-1 enzyme. It is produced via proteolytic cleavage of the full-length DMPK-1, which removes a C-terminal hydrophobic membrane association domain, converting the membrane-bound kinase into a smaller (~71-80 kDa) soluble protein. It features a Ser/Thr protein kinase catalytic domain and a coiled-coil domain. The coiled-coil domain is essential for forming dimers and higher-order oligomers, which enhances catalytic activity. Proteolytic processing to DMPK2 also increases the catalytic activity approximately 3-fold by removing an autoinhibitory domain at the carboxy terminus. It is hypothesized to function as a downstream effector of CDC42 during cytoskeletal rearrangement. Agonist binding to the phorbol ester binding site disrupts this, releasing the kinase domain to allow N-terminus-mediated dimerization and kinase activation by transautophosphorylation. It is maintained in an inactive, closed conformation by an interaction between the kinase domain and the negative autoregulatory C-terminal coiled-coil region. DMPK2 also plays a role in the regulation of actin-myosin contractility through the modulation of MYPT1 and MLC2 activity, which are required for cell invasion and motility. The DMPK2 protein is highly expressed in cardiac, smooth, and skeletal muscle, with little to no expression in the brain, placenta, lung, liver, kidney, and pancreas. The DMPK gene is associated with Myotonic Dystrophy type 1 (DM1), where a CTG repeat expansion leads to a "toxic gain-of-function" RNA that causes the disease, while a decrease in normal DMPK protein levels may also contribute to the pathology. The DMPK2 gene maps to chromosome 11q13. This description may include information annotated by UniProt and/or Google AI.