Product Name:
CDKL3-BCT
Product Number:
ab-nk262-1
Target Full Name: Cyclin-dependent kinase-like 3; Serine/threonine protein kinase NKIAMRE
Target Alias: Serine/threonine protein kinase NKIAMRE
Product Type Specific: CDKL3 (NKIAMRE) protein kinase pan-specific antibody
Antibody Code: NK262-1
Antibody Target Type: Pan-specific
Protein UniProt: Q8IVW4
Protein SigNET: CDKL3
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CDKL3 sequence peptide Cat. No.: PE-01BCO99
Antibody Immunogen Sequence: CANENKRKLNFSRSDRKEFH
Antibody Immunogen Description: Corresponds to amino acid residues A505 to H523; Pre-C-terminus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Rat | Mouse | Frog | Zebra fish.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 57-67 kDa.
Related Product 1: CDKL3 (505-523) antibody blocking peptide (Cat. No.: PE-01BCO99)
Related Product 2: CDKL2 expression antibody (Cat. No.: AB-NK261-1)
Related Product 3: CDKL5 Y171 phosphorylation antibody (Cat. No.: AB-PK576)
Scientific Background: CDKL3 (Cyclin-dependent kinase-like 3; NKIAMRE) is a protein-serine/threonine kinase of the CMGC group and CDKL family. Endogenous CDKL3 is primarily found in the nucleus, allowing it to regulate cell cycle mechanisms, but it can also be found in the cytoplasm. CDKL3 contains a conserved putative cyclin-binding α-helix and a nuclear localization sequence (NLS), with a 455-amino acid protein structure. It is likely that phosphorylation at T158 and Y160 is required for catalytic activity and phosphorylation at Y15 is inhibitory based on homology with other CDK's. It seems to function as an oncogene, promoting cell cycle progression, proliferation, and cancer development. It acts as a driver of G1 phase advancement and quiescence exit by phosphorylating the retinoblastoma (Rb) protein and preventing the degradation of CDK4. It is often localized in the nucleus, where it plays a key role in regulating the G0/G1 transition, especially in cancers like gliomas, osteosarcomas, and hepatocellular carcinomas. Its activity often linked to promoting tumor growth, such as via the PI3K/Akt/mTOR pathway. The gene was found absent in leukemic and dysmyelopoiesis patients with chromosome 5q deletion. While CDKL3 is essential for cell cycle progression, knockdown commonly leads to G2 arrest or blocking the G0/G1 to S phase transition. CDKL3 is also involved in neuronal morphogenesis and is highly active in the proliferating cells surrounding the brain ventricle. It may play a role in Meckel Syndrome type 4 and Orofaciodigital Syndrome VI. This description may include information annotated by UniProt and/or Google AI.