Product Name:
CDKL2-CT
Product Number:
ab-nk261-1
Target Full Name: Cyclin-dependent kinase-like 2; Serine/threonine protein kinase KKIAMRE
Target Alias: Kinase CDKL2; KKIAMRE; P56; Protein kinase p56 KKIAMRE; Serine/threonine-protein kinase KKIAMRE
Product Type Specific: CDKL2 (KKIAMRE) protein kinase pan-specific antibody
Antibody Code: NK261-1
Antibody Target Type: Pan-specific
Protein UniProt: Q92772
Protein SigNET: CDKL2
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human CDKL2 sequence peptide Cat. No.: PE-01BCN85
Antibody Immunogen Sequence: CLSDDSGADLPQMEHQH
Antibody Immunogen Description: Corresponds to amino acid residues L478 to H493; C-terminus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 52-57 kDa.
Related Product 1: CDKL2 (478-493) antibody blocking peptide (Cat. No.: PE-01BCN85)
Related Product 2: CDKL3 expression antibody (Cat. No.: AB-NK262-1)
Related Product 3: CDKL5 Y171 phosphorylation antibody (Cat. No.: AB-PK576)
Scientific Background: CDKL2 (KKIAMRE) is a 56-81 kDa protein-serine/threonine kinase of the CMGC group and CDKL family. It is likely that phosphorylation at Tyr-161 is required for catalytic activity and phosphorylation at Tyr-15 is inhibitory based on homology with other CDK's. It is induced when cells are stimulated with epidermal growth factor. It may play a role in signal transduction and differentiation. It has been found to have a role in memory and learning, and its deficiency causes reduced fear responses in mice. CDKL2 is mainly localized in the cytoplasm, but also found in the nucleoplasm and centrosome. It is expressed in the brain (amygdala, cortex, hippocampus), testis, retina, lungs and kidneys. CDKL2 may be an oncoprotein (OP). It has been identified as a novel potent promoter for epithelial-mesenchymal transition and breast cancer progression. CDKL2 stimulated primary tumour formation and metastasis in a breast cancer xenograft model. It is expressed at significantly higher levels mesenchymal human breast cancer cell lines than in epithelial lines, and its over-expression/amplification in human breast cancers is correlated with shorter disease-free survival. This description may include information annotated by UniProt and/or Google AI.