Product Name:
AMPKa2-AKCD2
Product Number:
ab-nk260-1
Target Full Name: 5'-AMP-activated protein kinase catalytic subunit alpha-2
Target Alias: AAPK2; AMPK alpha-2 chain; Kinase AMPK-alpha2; AMPK2; PRKAA; PRKAA2 ENSG00000162409
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK260-1
Antibody Target Type: Pan-specific
Protein UniProt: P54646
Protein SigNET: P54646
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human AMPKa2 (PRKAA2) sequence peptide Cat. No.: PE-01BCD99
Antibody Immunogen Sequence: CGSFMDDSAMHIPPGLKP
Antibody Immunogen Description: Corresponds to amino acid residues G349 to P365; After catalytic domain in region not conserved with AMPKa1
Production Method: Corresponds to amino acid residues G349 to P365; After catalytic domain in region not conserved with AMPKa1
Antibody Modification: Protein kinase pan-specific antibody
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 60-65 kDa.
Related Product 1: AMPKa2-AKCD2 blocking peptide
Related Product 2: AMPKa1-AKCD pan-specific antibody (Cat. No.: AB-NK259-1)
Related Product 3: AMPKa2-pS377 phosphosite-specific antibody (Cat. No.: AB-PK522) http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK522
Related Product 4: AMPKSelectide - AMPKa1 (PRKAA1) protein kinase substrate peptide
Related Product 5: AMPKSubtide - AMPKa2 (PRKAA2) protein kinase substrate peptide
Related Product 6: AcCoACarbox (73-85) S77A, KinSub - Acetyl-Coenzyme A carboxylase alpha (H73-R85, mouse) peptide; AMPKA protein kinase substrate; SAMStide
Related Product 7: GSK3b (3-12) KinSub - Glycogen synthase kinase 3-beta (GSK3b) N-terminus (G3-E12, human) peptide; Akt protein kinase substrate peptide
Scientific Background: AMPKa2 (PRKAA2) is a protein-serine/threonine kinase of the CAMK group and CAMKL family. It is the catalytic subunit of AMP-activated protein kinase (AMPK),which is an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. It also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton. Binding of AMP results in allosteric activation, inducing phosphorylation on T172 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39.It is also activated by phosphorylation by CAMKK2 triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio. Defects in PRKAA2 are linked to Peutz-Jeghers Syndrome, which is a relatively rare autosomal dominant disease where benign polyps develop on the GI tract.