Product Name:
YSK4-1
Product Number:
ab-nk256-1
Target Full Name: SPS1/STE20-related protein kinase YSK4; Mitogen-activated protein kinase kinase kinase 8; MAP3K19
Target Alias: FLJ23074; Rck; Regulated in COPD, protein kinase; YSK4; YSK4 Sps1/Ste20-related kinase
Product Type Specific: YSK4 protein kinase pan-specific antibody
Antibody Code: NK256-1
Antibody Target Type: Pan-specific
Protein UniProt: Q56UN5
Protein SigNET: YSK4
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human YSK4 (MAP3K19) sequence peptide Cat. No.: PE-01AZK85
Antibody Immunogen Sequence: MSSMPKPERHAESLLC
Antibody Immunogen Description: Corresponds to amino acid residues M1 to L15; N-terminus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 0.5 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human | Chimpanzee | Rhesus macaque | Rat.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 50-70 kDa.
Antibody Specificity: High
Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human MEKK2 and MEKK6.
Related Product 1: YSK4 (1-15) antibody blocking peptide (Cat. No.: PE-01AZK85)
Related Product 2: YSK4-2 pan-specific antibody (Cat. No.: AB-NK256-2)
Related Product 3: YSK4-3 pan-specific antibody (Cat. No.: AB-NK256-3)
Related Product 4: YSK1 T174 phosphorylation antibody (Cat. No.: AB-PK859)
Scientific Background: YSK4 (MAP3K19; RCK) is an ~83 kDa protein-serine/threonine kinase of the STE group and STE11 family. It plays a crucial role in regulating cell survival, specifically by activating the ERK and JNK signalling pathways in cancers, particularly in KRAS-mutant lung cancer cells. It phosphorylates MAPK/ERK kinase (MEK) and MAPK kinase 7 (MKK7), driving pathway activation. YSK4 is a key survival factor for KRAS-mutant cancers and is often overexpressed in the lungs of patients with chronic obstructive pulmonary disease (COPD). This description may include information annotated by UniProt and/or Google AI.