Product Name:
WNK4-1
Product Number:
ab-nk255-1
Target Full Name: Serine/threonine-protein kinase WNK4
Target Alias: Kinase WNK4; PRKWNK4; Protein kinase with no lysine 4; Protein kinase, lysine-deficient 4; WNK lysine deficient protein kinase 4; PHA2B; CCDS11439.1; ENSG00000126562
Product Type Specific: WNK4 protein kinase pan-specific antibody
Antibody Code: NK255-1
Antibody Target Type: Pan-specific
Protein UniProt: Q96J92
Protein SigNET: WNK4
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human WNK4 (PRKWNK4) sequence peptide Cat. No.: PE-01AZH85
Antibody Immunogen Sequence: MLASPATETTVLMSQC
Antibody Immunogen Description: Corresponds to amino acid residues M1 to Q15; N-terminus.
Production Method: The immunizing peptide was produced by solid phase synthesis on a multipep peptide synthesizer and purified by reverse-phase hplc chromatography. Purity was assessed by analytical hplc and the amino acid sequence confirmed by mass spectrometry analysis. This peptide was coupled to KLH prior to immunization into rabbits. New Zealand White rabbits were subcutaneously injected with KLH-coupled immunizing peptide every 4 weeks for 4 months. The sera from these animals was applied onto an agarose column to which the immunogen peptide was thio-linked. Antibody was eluted from the column with 0.1 M glycine, pH 2.5. Subsequently, the antibody solution was neutralized to pH 7.0 with saturated Tris.
Antibody Modification: Unconjugated. Contact KInexus if you are interest in having the antibody biotinylated or coupled with fluorescent dyes.
Antibody Concentration: 1 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: This antibody detects the target protein in the following species due to conservation of amino acid sequence: Human.
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 110, 140-180 kDa.
Antibody Specificity: Medium
Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human WNK1.
Related Product 1: WNK4 (1-15) antibody blocking peptide (Cat. No.: PE-01AZH85)
Related Product 2: WNK4-3 pan-specific antibody (Cat. No.: AB-NK255-3)
Related Product 3: WNK1-1 pan-specific antibody (Cat. No.: AB-NK252-1)
Related Product 4: WNK1-2 pan-specific antibody (Cat. No.: AB-NK252-2)
Related Product 5: WNK2-1 pan-specific antibody (Cat. No.: AB-NK253-1)
Related Product 6: WNK2-2 pan-specific antibody (Cat. No.: AB-NK253-2)
Related Product 7: WNK2-3 pan-specific antibody (Cat. No.: AB-NK253-3)
Related Product 8: WNK3 expression antibody (Cat. No.: AB-NK254-1)
Related Product 9: WNK3 expression antibody (Cat. No.: AB-NK254-2)
Scientific Background: WNK4 (With-No-Lysine Kinase 4; PRKWNK4) is a protein-serine/threonine kinase of the Other group and WNK family. It regulates electrolyte homeostasis. It acts as an integrative regulator of ion transporters, notably the thiazide-sensitive \(\text{NaCl}\) cotransporter (NCC), promoting reabsorption and regulating K+ secretion via ROMK channels. WNK4 can activate the genes SCNN1A, SCNN1B, SCNN1D, SGK1, TRPV5 and TRPV6. The renal potassium channel KCNJ1, through a kinase-mediated mechanism, will be inhibited via clathrin-mediated receptor endocytosis. WNK4 can also phosphorylate NEDD4L. Activation of WNK4 requires autophosphorylation of Ser-335. Phosphorylation of Ser-331 also promotes increased activity. It lacks a conserved lysine in subdomain II of the catalytic domain, and uses a lysine in subdomain I instead. WNK4 is present in the tight junction complex of kidney cells, where it controls paracellular chloride transport and ion permeability. WNK4 activity is regulated by the KLHL3–Cullin-3 E3 ligase complex; impairments in this pathway increase WNK4 abundance, contributing to FHHt.It is highly expressed in the distal convoluted tubule (DCT) and cortical collecting duct (CCD). Familial Hyperkalemic Hypertension (FHHt): Gain-of-function mutations in WNK4 lead to excessive NCC activity, causing high blood pressure, hyperkalemia (high potassium), and hypercalciuria. This description may include information annotated by UniProt and/or Google AI.