Product Name:
WNK3-2
Product Number:
ab-nk254-2
Target Full Name: Serine/threonine-protein kinase WNK3
Target Alias: KIAA1566; PRKWNK3; Protein kinase, lysine-deficient 3; RP3-390O13_2; CCDS14357.1; ENSG00000196632
Product Type Specific: Protein kinase pan-specific antibody
Antibody Code: NK254-2
Antibody Target Type: Pan-specific
Protein UniProt: Q9BYP7
Protein SigNET: Q9BYP7
Antibody Type: Polyclonal
Antibody Host Species: Rabbit
Antibody Immunogen Source: Human WNK3 (PRKWNK3) sequence peptide Cat. No.: PE-01AZF80
Antibody Immunogen Sequence: CEEAEMKAVATSPSGR
Antibody Immunogen Description: Corresponds to amino acid residues E31 to R145;
Production Method: Corresponds to amino acid residues E31 to R145;
Antibody Modification: Protein kinase pan-specific antibody
Antibody Concentration: 0.3 / 0.25 mg/ml
Storage Buffer: Phosphate buffered saline pH 7.4, 0.05% Thimerasol
Storage Conditions: For long term storage, keep frozen at -40°C or lower. Stock solution can be kept at +4°C for more than 3 months. Avoid repeated freeze-thaw cycles.
Product Use: Western blotting | Antibody microarray
Antibody Dilution Recommended: 2 µg/ml for immunoblotting
Antibody Species Reactivity: Human
Antibody Positive Control: The observed molecular mass of the processed target protein on SDS-PAGE gels is reported to be around 170-250 kDa.
Antibody Specificity: Very high
Antibody Cross Reactivity: No immunoreactivity on protein dot blots with recombinant human WNK1.
Related Product 1: WNK3-2 blocking peptide
Related Product 2: WNK3-1 pan-specific antibody (Cat. No.: AB-NK254-1)
Related Product 3: WNK3-3 pan-specific antibody (Cat. No.: AB-NK254-3)
Scientific Background: WNK3 (PRKWNK2) is a protein-serine/threonine kinase of the Other group and WNK family. Activation requires autophosphorylation of S308. Phosphorylation of S304 also promotes increased activity. Interestingly, WNK3 lacks the catalytic lysine in subdomain II that is believed to be critical for phoshotransferase activity, and instead has a conserved lysine in subdomain I. It functions as a positive regulator of the transcellular Ca2+ transport pathway, and it plays a role in the increase of cell survival in a caspase-3-dependent pathway.
